We narrowed to 643 results for: pBR322

1. ABE8.8-m

   Plasmid

   #136294

   Purpose

   expresses ABE8.8-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.8-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ABE8.20-m

   Plasmid

   #136300

   Purpose

   expresses ABE8.20-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ABE8.17-m

   Plasmid

   #136298

   Purpose

   expresses ABE8.17-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.17-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ABE8.13-m

   Plasmid

   #136296

   Purpose

   expresses ABE8.13-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.13-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T7_CC_PE7_IVT_Template

   Plasmid

   #223022

   Purpose

   Template for in vitro transcription of PE7. For HSPC-related experiments.

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-dCas13-inactive M3M14nes

   Plasmid

   #157855

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

   Plasmid

   #106309

   Purpose

   The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
   

   Use

   Tags

   6xflag-3xHaloTag(MtoL)

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ABE8.20-d

   Plasmid

   #136301

   Purpose

   expresses ABE8.20-d in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-d
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. OPEN Beta2-microglobulin

    Plasmid

    #215657

    Purpose

    E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN Beta2-microglobulin (B2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Histidine 31 to Cysteine

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ABE8.17-d

    Plasmid

    #136299

    Purpose

    expresses ABE8.17-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE8.8-d

    Plasmid

    #136295

    Purpose

    expresses ABE8.8-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ABE8.13-d

    Plasmid

    #136297

    Purpose

    expresses ABE8.13-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD2_miniluc_newP7

    Plasmid

    #174105

    Purpose

    adding the mini luciferase and mini promoter to the MPRA lenti construct

    Depositor

    Paul Khavari

    Article

    Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

    Insert

    mini luciferase mini-promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6-sgRNA-hcr3

    Plasmid

    #193660

    Purpose

    Expression of tandem pre-sgRNA array hcr3 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. U6-sgRNA-hcr5

    Plasmid

    #193661

    Purpose

    Expression of tandem pre-sgRNA array hcr5 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. U6-sgRNA-pcr4

    Plasmid

    #193662

    Purpose

    Expression of tandem pre-sgRNA array pcr4 for LbCas12a

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSuiAb

    Plasmid

    #226171

    Purpose

    multiple resistance marker with apramycin and kanamycin

    Depositor

    Woojun Park

    Insert

    apramycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBR322ori

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pig3 Promoter

    Plasmid

    #16489

    Depositor

    Bert Vogelstein

    Article

    Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

    Insert

    PIG3 promoter (TP53I3 Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only