We narrowed to 6,573 results for: tac

1. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEVA26_sgRNA-A1_M13ps

   Plasmid

   #231414

   Purpose

   M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-A1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA63_sgRNA-B0_M13ps

   Plasmid

   #231421

   Purpose

   M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-B0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVA19_sgRNA-C1_M13ps

   Plasmid

   #231411

   Purpose

   M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVA19_sgRNA-C0_M13ps

   Plasmid

   #231410

   Purpose

   M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWP89

   Plasmid

   #61264

   Purpose

   IncP-based replicating vector expressing dTomato under tac promoter.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Insert

   Ptac-dTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJB158

   Plasmid

   #202601

   Purpose

   RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DT035A

   Plasmid

   #159397

   Purpose

   Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.tac-sfGFP
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Endophilin II Full

   Plasmid

   #47409

   Depositor

   Peter McPherson

   Insert

   Endophilin II
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

10. Endophilin III Full

    Plasmid

    #47410

    Depositor

    Peter McPherson

    Insert

    Endophilin III
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

11. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDA03

    Plasmid

    #65943

    Purpose

    Theophylline riboswitch D derivatives 03

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDA08

    Plasmid

    #65944

    Purpose

    Theophylline riboswitch D derivatives 08

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTMR-S02

    Plasmid

    #65941

    Purpose

    TMR Riboswitch S02 with luciferase gene

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. DT033A

    Plasmid

    #159394

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAL-p5X

    Plasmid

    #150814

    Purpose

    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Type

    Empty backbone

    Use

    Tags

    malE A313V

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pOpen_taq

    Plasmid

    #153315

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    Drew Endy

    Insert

    Taq polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHN764

    Plasmid

    #178819

    Purpose

    Expression of EndoH in E. coli

    Depositor

    Kazuhiro Abe

    Insert

    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    

    Use

    Tags

    HIs8 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGTvL1-SGC

    Plasmid

    #39188

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Nicola Burgess-Brown

    Type

    Empty backbone

    Use

    Tags

    GST and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only