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pDONR223_SMAD4_p.G386V

Plasmid

#82772

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

G386V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB31_WT_V5

Plasmid

#82993

Purpose

Gateway Donor vector containing RAB31, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RAB31 (RAB31 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.E4K

Plasmid

#82860

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

E4K; E849D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.Y580F

Plasmid

#82834

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

Y580F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-Nppa-Cre-P2A-TagRFP

Plasmid

#247328

Purpose

Expresses Cre and TagRFP under the control of the Nppa promoter.

Depositor

William Pu, Mason Sweat

Article

Sweat et al Nat Cardiovasc Res. 2023 Oct;2(10):881-8

Insert

Cre-P2A-TagRFP

Use

AAV

Expression

Mammalian

Promoter

Nppa proximal promoter

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GeNL_SS

Plasmid

#244128

Purpose

Sensor scaffold luciferase derived from Oplophorus luc.

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

GeNL_SS

Expression

Mammalian

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-CaBLAM_294W

Plasmid

#244130

Purpose

Lower affinity variant of CaBLAM bioluminescent calcium indicator

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM_294W

Expression

Mammalian

Mutation

Calmodulin N98W

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-CaBLAM_332W

Plasmid

#244131

Purpose

Higher affinity variant of CaBLAM bioluminescent calcium indicator

Depositor

Nathan Shaner

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM_332W

Expression

Mammalian

Mutation

Calmodulin Q135W

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV EfIa DIO CaBLAM

Plasmid

#244229

Purpose

Cre dependent bioluminescent reporter for calcium signaling

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-Nppa-Cre

Plasmid

#247326

Purpose

Expresses Cre recombinase under the control of the Nppa Promoter.

Depositor

William Pu, Mason Sweat

Article

Sweat et al Nat Cardiovasc Res. 2023 Oct;2(10):881-8

Insert

Nppa-Cre

Use

AAV

Expression

Mammalian

Mutation

TagRFP is out of frame and therefore not translat…

Promoter

Nppa Proximal promoter

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA mutant

Plasmid

#244882

Purpose

In vitro transcription of mutant human FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

GRCh38, chr2:70300842G>A

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA WT

Plasmid

#244883

Purpose

In vitro transcription of wild-type FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-mutant-3xFLAG-hygro

Plasmid

#244879

Purpose

Overexpression of mutant human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

GRCh38, chr2:70300842G>A

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg2

Plasmid

#244864

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg1

Plasmid

#244863

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg2

Plasmid

#244852

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg1

Plasmid

#244851

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-9c)

Plasmid

#241053

Purpose

Expresses SARS-CoV-2 spike (B.1-9c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-9c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,R158A,T478K,E484P,F490R,S494R,K529L,D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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