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pCMV-NHERF1-PDZ1iP

Plasmid

#28295

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 1-148 (deletion of PDZ2 and EB domain…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
mCas13 Twin-SUMO

Plasmid

#199753

Purpose

Expresses codon optimized miniature mCas13 for protein expression in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

Insert

mCas13

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-ADRB2-mNG

Plasmid

#195145

Purpose

Image recycling cargo

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

ADRB3 (ADRB3 Human)

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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HA-hDAGLb-V5

Plasmid

#87675

Purpose

Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Insert

Human Diacylglycerol Lipase Beta (DAGLB Human)

Tags

HA and V5 tag

Expression

Mammalian

Mutation

Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-PDZ2

Plasmid

#28296

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 148-298 (deletion of the first PDZ do…

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTorPE-G-GECO1.1

Plasmid

#32467

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.1

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmEGFP-C

Plasmid

#113751

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

3xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-N

Plasmid

#113754

Purpose

Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFGL1252_TetGFP(Hyg)

Plasmid

#118993

Purpose

TetOFF controlled GFP-tagging vector with Hygromycin selection

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression (in magnaporthe oryzae)

Promoter

Ustilago maydis Mfa1 basal promoter with six tetO…

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mEGFP-C

Plasmid

#113749

Purpose

Gateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mRuby-C

Plasmid

#113755

Purpose

Gateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-C

Plasmid

#113757

Purpose

Gateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-THP-Synaptophysin-pHluorin

Plasmid

#242903

Purpose

Fluorescent reporter for imaging of synaptic vesicle recycling in Dopaminergic Neurons

Depositor

Timothy Ryan

Article

Pulido et al Proc Natl Acad Sci U S A. 2025 Dec 16;12

Insert

synaptophysin plus pHluorin

Use

AAV

Tags

mouse synaptophysin

Expression

Mammalian

Promoter

rat Tyrosine hydroxylase

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 hygro sgmPOLE_2

Plasmid

#241443

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_2 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 hygro sgmPOLE_1

Plasmid

#241442

Purpose

Delete mouse Pole

Depositor

Richard Possemato

Insert

sgmPOLE_1 (POLE Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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GMR11F02-Gal4 UAS-GFP

Plasmid

#230907

Purpose

Tissue-specific expression of GFP in wing and haltere imaginal discs.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Expression

Insect

Promoter

GMR11F02Gal4-UAS

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Tags

Myc-6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-Nanobody-C5

Plasmid

#209420

Purpose

Express anti-SARS-CoV-2-Nanobody C5 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-Nanobody-C5

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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