We narrowed to 14,132 results for: flag

1. pMXS-IRES-BLAST flag-CAD P1375A P1376A

   Plasmid

   #188146

   Purpose

   Expresses C-terminal flag-tagged human CAD P1375A P1376A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193317

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Tags

   4FLAG

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397C_ABE

   Plasmid

   #189648

   Purpose

   psa A Right _G1397C_ABE

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

   Insert

   TALE and DddAtox and TadA 8e
   

   Use

   TALEN

   Expression

   Plant

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 C-ABE

   Plasmid

   #189650

   Purpose

   rbc L 294 Left_G1397 C-ABE

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

   Insert

   TALE and DddAtox and TadA 8e
   

   Use

   TALEN

   Expression

   Plant

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 N

   Plasmid

   #189651

   Purpose

   rbc L 294 Right_G1397 N

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

   Insert

   TALE and DddAtox
   

   Use

   TALEN

   Expression

   Plant

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

   Plasmid

   #158771

   Purpose

   Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
   

   Use

   Lentiviral

   Mutation

   Flexible linkers, ADVVCC/QMSY cleavage site

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA

   Plasmid

   #164796

   Purpose

   pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   Flag

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJEP209-Lenti-1.3CaMKII P-Intron-Flag-GluN2A-WPRE-pA

   Plasmid

   #74714

   Purpose

   pLenti vector backbone designed to express Flag-GluN2A from a 1.3CaMKII promoter. Transgene in reverse orientation.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Flag-GluN2A
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   1.3CaMKIIa

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC-Flag-BirA-Linker-Myc-mRap1-I312R

   Plasmid

   #184645

   Purpose

   Retroviral expression of BirA Rap1 I312R

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   BirA-rap1-I312R
   

   Use

   Retroviral

   Tags

   BirA FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

    Plasmid

    #182380

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mito-mTFP1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    CMV promoter and SV40 promoter

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

    Plasmid

    #175427

    Purpose

    Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E55A E57A K58A
    

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E55A E57A K58A

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

    Plasmid

    #167199

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R S364L
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-2xFLAG-2xSTREP-CCND1(T286I)-IRES-mCherry

    Plasmid

    #172638

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ile

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry

    Plasmid

    #172636

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Leu

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Flag-Polß(L301R/V303R)-IRES-neo

    Plasmid

    #128664

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R) protein with neomycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid

    #136884

    Purpose

    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    engrailed repressor domain (en Fly)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO-FUW-FLAG-NLS-VP64-MCS_N-term

    Plasmid

    #136886

    Purpose

    Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    VP64 activator domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

    Plasmid

    #136888

    Purpose

    Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

    Plasmid

    #167200

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R D421N
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

    Plasmid

    #167198

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E294K
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge