We narrowed to 7,534 results for: ski

1. pLVX-FFSS-Cyclin D1 (T286A)

   Plasmid

   #215152

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Mutation

   With T286A mutation.

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPaGE Pyl TAG FliC T248TAG

   Plasmid

   #160089

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

   Inserts

   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Flagellin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed Threonine 248 to TAG stop-codon.

   Promoter

   Pseudomonas aeruginosa Leu-tRNA native promoter a…

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. anti-Desmin_D7-pBIOCAM5

   Plasmid

   #39346

   Depositor

   John McCafferty

   Article

   Schofield et al Genome Biol. 2007;8(11):R254.

   Insert

   scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)
   

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-hSOX2-17

   Plasmid

   #193350

   Purpose

   Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2-17 (SOX2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   5'LTR

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_CTNNB1_p.S45F

   Plasmid

   #82821

   Purpose

   Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Gateway entry vector

   Mutation

   S45F

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304-ARNT-d414

   Plasmid

   #203579

   Purpose

   Expresses a functionally inactive version of ARNT with 5’ 414 bp deletion [Δ414] in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

   Insert

   ARNT (ARNT Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   5' 414 basepair deletion [Δ414]

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

   Plasmid

   #208292

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpCas9(D10A)

   Promoter

   CMV and T7

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-TARDBP-A315T

   Plasmid

   #141324

   Purpose

   Gateway cloning of TARDBP-A315T

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-A315T (TARDBP Human)
   

   Use

   Gateway cloning

   Mutation

   A315T

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_MAX_WT

   Plasmid

   #82888

   Purpose

   Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   MAX (MAX Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHR-EYFP-hHP1β

    Plasmid

    #103829

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-hHP1β (CRY2, CBX1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIRTS-FTO-control

    Plasmid

    #216859

    Purpose

    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-FTO-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Promoter

    Syn1, U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1e-CDK12/Cyclin K

    Plasmid

    #231730

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG1e-CDK13/Cyclin K

    Plasmid

    #231731

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK13 (CDK13 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p21-HA(RxL)-NeoR

    Plasmid

    #215106

    Purpose

    Expresses p21-HA(RxL) in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIZ GFP PODXL delta PBM + Ezrin

    Plasmid

    #202418

    Purpose

    Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    Ezrin and GFP

    Mutation

    delete PDZ binding motif

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmiRA1-MYC

    Plasmid

    #141121

    Purpose

    Lentiviral overexpression of MYC in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-PGK-RAP2A-WT-neo

    Plasmid

    #156177

    Purpose

    Lentiviral vector for expression of RAP2A-WT, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-PE SpRY

    Plasmid

    #179082

    Purpose

    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

    Promoter

    CAG

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-2dV-Camui (T305D/T306D)

    Plasmid

    #220368

    Purpose

    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed both Threonine 305 and 306 to aspartic ac…

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_MAPK7_WT

    Plasmid

    #82925

    Purpose

    Gateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    NM_002749.3 (MAPK7 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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