We narrowed to 10,433 results for: yeast

Showing: 9631 - 9660 of 10433 results

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pHyg-CUbo-AID*-GFP

Plasmid

#212770

Purpose

C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K48R/K63R)

Plasmid

#212765

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K63R)

Plasmid

#212764

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K48R)

Plasmid

#212763

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo

Plasmid

#212762

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-3xFLAG-Ubc7

Plasmid

#212801

Purpose

Constitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Ubc13

Plasmid

#212806

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Mms2

Plasmid

#212807

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3243

Plasmid

#210187

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33P-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33P

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3176

Plasmid

#210178

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-G141S-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

G141S

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3145

Plasmid

#210169

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-D147V-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

D147V

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3139

Plasmid

#210167

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33G-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33G

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3142

Plasmid

#210168

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-D147G-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

D147G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRH3175

Plasmid

#210177

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)

Expression

Yeast

Mutation

R33G,G141S

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C1

Plasmid

#141248

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Reduced Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPG-GPD-TRPC

Plasmid

#190113

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.108

Plasmid

#184977

Purpose

Express -Eco1 LYP1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12

Expression

Yeast

Mutation

LYP1 donor E27stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.106

Plasmid

#184975

Purpose

Express -Eco1 CAN1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12

Expression

Yeast

Mutation

CAN1 donor G444stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.94

Plasmid

#184985

Purpose

Express -Eco1 RT/Cas9 fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker2-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.037

Plasmid

#184966

Purpose

Express -Eco1 dead RT and ncRNA in yeasst

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT

Expression

Yeast

Mutation

Human codon optimized RT, YXDD catalytic region m…

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.71

Plasmid

#184983

Purpose

Express -Eco1 RT/Cas9 fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1RT-fusion_linker1-Cas9

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL.112

Plasmid

#184981

Purpose

Express -Eco1 FAA1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12

Expression

Yeast

Mutation

FAA1 donor P233stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.002

Plasmid

#184972

Purpose

Express -Eco1 ADE2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12

Expression

Yeast

Mutation

ADE2 donor P272stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.110

Plasmid

#184979

Purpose

Express -Eco1 TRP2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12

Expression

Yeast

Mutation

TRP2 donor E64stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMM4_14L

Plasmid

#183063

Purpose

Plasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Inserts

mCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

RET2, Synthetic promoter, and TDH3

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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