We narrowed to 9,894 results for: UTY

1. pIND4-GFP-MmXYRS

   Plasmid

   #222745

   Purpose

   Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker

   Depositor

   Steven Boxer

   Inserts

   sfGFP
   MmXYRS
   Mm Pyl tRNA
   

   Use

   Tags

   6xHis

   Expression

   Mutation

   Contains the following substitutions: V346A, W348…

   Promoter

   rrnB

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKS_3HA_PAC

   Plasmid

   #122564

   Purpose

   Vector for endogenously tagging Giardia genes with a triple HA tag (Puromycin selection)

   Depositor

   Alexander Paredez, Zacheus Cande

   Article

   Gourguechon et al Eukaryot Cell. 2011 Jan;10(1):142-5. doi

   Type

   Empty backbone

   Use

   Tags

   hemagglutinin tag

   Expression

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKS_3HA_BSR

   Plasmid

   #122567

   Purpose

   Vector for endogenously tagging Giardia genes with a triple HA tag (Blasticidin selection)

   Depositor

   Alexander Paredez, Zacheus Cande

   Article

   Gourguechon et al Eukaryot Cell. 2011 Jan;10(1):142-5. doi

   Type

   Empty backbone

   Use

   Tags

   3x hemagglutinin tag

   Expression

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB2H12*

   Plasmid

   #163838

   Purpose

   Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12 (Y64A)

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   PTPN12 Y64A

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pB2H12

   Plasmid

   #163837

   Purpose

   Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   PTPN12 E57D

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB2H6*

   Plasmid

   #163836

   Purpose

   Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1 (R459M)

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   SHP1 R459M

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB2Hopt*

   Plasmid

   #163831

   Purpose

   Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B(C215S)

   Depositor

   Jerome Fox

   Insert

   SpecR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   PTP1B C215S

   Promoter

   Pro1;ProD;pLacZ-Opt

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pANY4-pR18/pL18-ccdB

   Plasmid

   #164899

   Purpose

   Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.

   Depositor

   Yingfeng An

   Insert

   ccdB cassette
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK573

   Plasmid

   #72456

   Purpose

   Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   synthetic gene with ~57 silent substitutions

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK569

    Plasmid

    #72455

    Purpose

    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~51 silent substitutions

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSBBi-RP TCS1

    Plasmid

    #139161

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    Caffeine Synthase 1 [Camellia sinensis]
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBBi-RP CaDXMT

    Plasmid

    #139165

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    3,7-dimethylxanthine N-methyltransferase 1 [Coffea arabica]
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBBi-RP CsAncCS

    Plasmid

    #139167

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    Predicted Camellia sinensis Ancestral Caffeine Synthase
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBBi-RP PcAncCS2

    Plasmid

    #139170

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2); the other side contains RFP and puromycin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    Predicted Paullinia cupana var sobilis Ancenstral Caffeine Synthase-2
    

    Use

    Transposon

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBBi-RP CaMXMT

    Plasmid

    #139172

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    monomethylxanthine methyltransferase 1 [Coffea arabica]
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBbi-RP Hu CXCR3

    Plasmid

    #183255

    Purpose

    Expression of human CXCR3 in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Insert

    CXCR3 (CXCR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZE27GFP

    Plasmid

    #75452

    Purpose

    Plasmid for constitutive expression of gfp

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M3-PAmcherry1

    Plasmid

    #114189

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Use

    Tags

    3xHA and PAmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB6K-mcherry-iATPsnFR1.1

    Plasmid

    #227239

    Purpose

    Inducible ATP sensor for Pseudomonas putida

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge