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DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)

Plasmid

#73476

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-GFP-P2A-N2c(G)

Use

AAV

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_TNIK

Plasmid

#45276

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

TNIK (TNIK Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-erHRP(N175S mutant)

Plasmid

#79909

Purpose

erHRP(N175S mutant) pCMV backbone

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO

Plasmid

#73471

Purpose

Expresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry-P2A-FlpO

Use

Neurotropic virus

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABECD

Plasmid

#66536

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pENTRY-YFP

Plasmid

#15302

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

YFP

Use

Gateway entry

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
Sf-U7snRNA-ESE-AS-NsiI

Plasmid

#190694

Purpose

Basic vector used for the expression of U7-based antisense oligonucleotides

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U7 promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(+)-Fc

Plasmid

#59314

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox2

Plasmid

#213726

Purpose

Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox2 (Rbfox2 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pUCHR-inmCherry

Plasmid

#60239

Purpose

HIV-1 transfer vector that encodes mcherry reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inmCherry

Use

Lentiviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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p1553 CMV-EBNA1 (delta GGA)

Plasmid

#26648

Depositor

Bill Sugden

Article

Aiyar et al J Biol Chem. 1998 Dec 4. 273(49):33073-8

Insert

Epstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)

Expression

Mammalian

Mutation

deletion of 224 amino acids of EBNA-1 (aa101-324 …

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-sfGFP

Plasmid

#116917

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

Plasmid

#53959

Purpose

allows Cre-mediated expression of transgene

Depositor

Jeffrey Robbins

Article

Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

Type

Empty backbone

Use

Cre/Lox

Tags

Lox-CAT-Lox

Expression

Mammalian

Promoter

CMV enhancer, chicken actin promoter

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)

Plasmid

#73478

Purpose

Encodes the G protein to enable transsynaptic RAV infection

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

H2B-3XHA-P2A-N2c(G)

Use

AAV

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-eGFP

Plasmid

#157734

Purpose

mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-eGFP

Tags

Flag-His8

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKB33 (pUC19-KANMX-tetO7-CYCpr)

Plasmid

#210472

Purpose

Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Insert

KanMX-TetO7-CYCpr cassette

Use

Synthetic Biology

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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