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pOPTM p62 PB1 (1-122)

Plasmid

#210428

Purpose

Bacterial expression of MBP-PB1(1-122) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-122) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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myr-palm-Gamillus(2x)

Plasmid

#197921

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Expression

Mammalian

Mutation

none

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-mGreenLantern(2x)

Plasmid

#197917

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern-mGreenLantern

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-Gamillus(2x)

Plasmid

#197915

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABmNeonC

Plasmid

#178464

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmNeon, TatC

Tags

mNeonGreen fused to TatB

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatAkBxCs

Plasmid

#178544

Purpose

pTatABC with KpnI, XbaI, and KpnI restriction sites after TatA, TatB, and TatC, respectively.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_TatABmCherryC

Plasmid

#178545

Purpose

pTatABC with an mCherry fluorescent protein tag at the end of TatB.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmCherry, TatC

Tags

mCherry fused to TatB

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-degFusionRed

Plasmid

#199463

Purpose

Arabinose-induced expression of the photodegradable His-tagged protein degFusionRed in E. coli.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

degFusionRed

Tags

6xHis

Expression

Bacterial

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-Gamillus

Plasmid

#197920

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-mut-siRNAres_I

Plasmid

#146587

Purpose

Mammalian Expression of HsSmg6-mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

HsSmg6-mut-siRNAres (SMG6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1mut-siRNAres_E

Plasmid

#146238

Purpose

Insect Expression of DmSMG6-PINNOB1mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-PINNOB1mut-siRNAres (Smg6 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1-siRNAres_E

Plasmid

#146239

Purpose

Insect Expression of DmSMG6-PINNOB1-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-PINNOB1-siRNAres (Smg6 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSMG6-siRNAres-V5His6_E

Plasmid

#146230

Purpose

Insect Expression of DmSMG6-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

DmSMG6-siRNAres (Smg6 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-5.1

Plasmid

#190589

Purpose

Lentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cells

Depositor

Jakob Reiser

Article

Bialkowska et al BMC Genomics. 2005 Sep 4;6:113. doi: 10.

Inserts

NeoR transgene
artificial exon encoding BSD and EGFP

Use

Lentiviral

Promoter

Cellular and SV40

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTorD-H6

Plasmid

#169039

Purpose

Escherichia coli TorD with C-terminal 6xHis in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

TorD-H6

Tags

6xHis

Expression

Bacterial

Mutation

The mutation N at position 46 was changed back to…

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3998rc

Plasmid

#184851

Purpose

A retroviral vector with blasticidin resistance for cloning inserts between loxP sites

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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