We narrowed to 11,757 results for: pcdna

1. pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

   Plasmid

   #200980

   Purpose

   Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Use

   Tags

   IgG1 Fc

   Expression

   Mammalian

   Mutation

   Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3_SP-GCGR-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194377

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 H2B-mIFP T2A Mpro (C145A)

   Plasmid

   #163080

   Purpose

   Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Inactive Mpro mutant C145A and H2B-mIFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3341 pcDNA3 Bcl-2 T69A S70A S87A

   Plasmid

   #13340

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 69 to Alanine; changed Serine 7…

   Promoter

   Available since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

   Plasmid

   #176060

   Purpose

   Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K164A and H165A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. (318) pcDNA3.1-myc-OGA(1-400)D174N

   Plasmid

   #168096

   Purpose

   Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   myc-OGA(1-400, D174N)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D174N catalytically inactive OGA

   Promoter

   T7/CMV

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-TAT-1-101-FLAG basic mutant

   Plasmid

   #35985

   Depositor

   Susana Valente

   Article

   Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

   Insert

   transactivating protein (tat )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   tat from HIV1, 101 aa; R to A mutations at aa 56,…

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

   Plasmid

   #136603

   Purpose

   Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   FKBP-C-mSOG-J-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

   Plasmid

   #166804

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S1213A

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Ras-LOCKR-S R89L (negative control)

    Plasmid

    #216497

    Purpose

    FRET-based reporter for Ras activity, untargeted, negative control

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S R89L negative control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid

    #125796

    Purpose

    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L (lose of function mutant)-mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid

    #164845

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression. Has N5…

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-S28 histone 3 phosphorylation reporter-NLS

    Plasmid

    #32787

    Depositor

    Alice Ting

    Article

    Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

    Insert

    CFP-14-3-3tau-H3 peptide-YFP
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD

    Plasmid

    #102606

    Purpose

    Mammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta K173-I220 and delta H390-C405

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CFP(159-238)-beta-5 in pcDNAI/Amp

    Plasmid

    #55763

    Purpose

    A carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    CFP(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
    

    Use

    Tags

    CFP(159-238) was fused to the amino terminus of G…

    Expression

    Mammalian

    Mutation

    CFP(159-238) includes a substitution of His for A…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

    Plasmid

    #83456

    Purpose

    Human PC1-CTp30 expression cassette with a GST-tag (678)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

    Plasmid

    #163990

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1461R

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1357 pcDNA3 flag FKHR AAA delta DB

    Plasmid

    #10696

    Depositor

    William Sellers

    Insert

    FKHR AAA deltaDB (FOXO1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    T24A S256A S319A , deletion in aa 208-220

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. IFI16-(192-393)-HinA (pcDNA3-FLAG)

    Plasmid

    #35062

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Contains HinA domain: aa 192-393

    Promoter

    CMV

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only