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pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Publication

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Expression

Bacterial

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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hSgk1_v3 Flag

Plasmid

#86039

Purpose

full length human Sgk1_i3 with Flag tag

Depositor

Christie Thomas

Publication

Raikwar et al Am J Physiol Renal Physiol. 2012 Dec 1;3

Insert

Alternate transcript v3 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v3; S313G

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Publication

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Expression

Bacterial

Promoter

T7

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(1004)

Plasmid

#44365

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(1004) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B16-TEF(203)

Plasmid

#44377

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(203) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(203)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCnCas12f1HS_sgRNA_MS13_empty

Plasmid

#204999

Purpose

CnCas12f1 with MS13 sgRNA site for Human cell genome editing

Depositor

Quanjiang Ji

Publication

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Type

Empty backbone

Use

CRISPR

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcsII-pb6-flag

Plasmid

#86770

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 6 (PSMB6 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

unknown

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Publication

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR223-LMP1

Plasmid

#195805

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP1

Depositor

Eric Johannsen

Publication

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP1 (LMP-1 Epstein-Barr virus)

Use

Gateway Cloning

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCG-TPMV Hd32-IL-13

Plasmid

#163869

Purpose

Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

Depositor

Jakob Reiser

Publication

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TPMV H glycoprotein/IL-13

Tags

Human IL-13

Expression

Mammalian

Mutation

Amino acids 2 to 33 at N terminus deleted

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1 S449A/T450A/S454A

Plasmid

#19847

Depositor

Ron Prywes

Publication

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Publication

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mhc2dab:GFP-LT

Plasmid

#68996

Purpose

for making transgenic fish

Depositor

David Traver

Publication

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

GFP-LT

Promoter

mhc2dab

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGST1-yHse1-UIM

Plasmid

#44429

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yHse1-UIM (150-190) (HSE1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-tUIM

Plasmid

#44428

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yVps27 tandem UIM (245-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-UIM2

Plasmid

#44427

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Vps27-UIM2 (290-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-STAM1-UIM

Plasmid

#44425

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

STAM1-UIM (STAM Human)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yGGA2-VHS

Plasmid

#44422

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA2 (1-169) (GGA2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yGGA1-VHS

Plasmid

#44421

Depositor

James Hurley

Publication

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA1-VHS (GGA1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only