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NBla_1.2_EmrE_TMD4_3P_G90V_G97V

Plasmid

#207847

Purpose

BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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NBla_1.2_EmrE_TMD4_3P

Plasmid

#207846

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in NBLa 1.2 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P_G90V_G97V

Plasmid

#207849

Purpose

BLaTM-System; Antiparallel negativecontrol EmrE_TMD4_3P_G90V_G97V in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mTB3-FLAG tag

Plasmid

#210204

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

FLAG-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mTB3-AviD tag

Plasmid

#210203

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

AviD-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMP123

Plasmid

#207950

Purpose

GreenBraid reverse destination vector pGBY2r - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMP122

Plasmid

#207948

Purpose

GreenBraid reverse destination vector pGBY1r - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMP075

Plasmid

#207952

Purpose

GreenBraid joker destination vector pGBY2j - Eco31I - dummy - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP074

Plasmid

#207951

Purpose

GreenBraid joker destination vector pGBY1j - Eco31I - dummy - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP072

Plasmid

#207922

Purpose

GreenBraid joker destination vector pGBZ1j - PaqCI - dummy - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMP073

Plasmid

#207923

Purpose

GreenBraid joker destination vector pGBZ2j - PaqCI - dummy - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP120

Plasmid

#207919

Purpose

GreenBraid reverse destination vector pGBZ1r - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMP121

Plasmid

#207921

Purpose

GreenBraid reverse destination vector pGBZ2r - PaqCI - Eco31I - spisPink- Eco31I - PaqCII

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-sTagRFP-Zeo

Plasmid

#207734

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-sTagRFP

Plasmid

#207701

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-C-sTagRFP-Blast

Plasmid

#207712

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Puro-sTagRFP

Plasmid

#207690

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pETSyt1C2AB(80-421)msGFP

Plasmid

#209009

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(96-421)

Plasmid

#209006

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 96-421 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80-142)

Plasmid

#209012

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge