We narrowed to 11,757 results for: pcdna

1. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

   Plasmid

   #83453

   Purpose

   Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   polycystin-1 (Pkd1 Mouse)
   

   Use

   Tags

   2xMyc, 3xFlag, and 6xHis

   Expression

   Mammalian

   Mutation

   S4144D

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

   Plasmid

   #194750

   Purpose

   To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. 9E10.mCHOP(S78&81A).pCDNA1/Amp

   Plasmid

   #21902

   Depositor

   David Ron

   Insert

   CHOP (Ddit3 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S78A S81A

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

   Plasmid

   #181814

   Purpose

   Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc5b D1 (Muc5b Mouse)
   

   Use

   Tags

   TEV cleavage site followed by 6xHistidine tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

   Plasmid

   #70668

   Purpose

   Human mutant of E2F1 lacking amino acids 2 to 126

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 lacking residues 2 to 126 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   lacks amino acids 2-126

   Promoter

   cmv

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. 1129 pcDNA3 GFP FKHR H215R AAA

   Plasmid

   #9034

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR H215R AAA (FOXO1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H215R T24A S256A S319A

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

8. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

   Plasmid

   #160742

   Purpose

   Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4,K852A)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. (282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

   Plasmid

   #160741

   Purpose

   Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nGFP-(EAAAK)4-OGT(4,K852A)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

    Plasmid

    #166798

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    LL100EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

    Plasmid

    #115843

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx-Q76A
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Q76A

    Promoter

    CMV

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid

    #59826

    Purpose

    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-myc-ΔD-box-CycA-GFP (525)

    Plasmid

    #39856

    Depositor

    Jonathon Pines

    Article

    den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

    Insert

    Cyclin A2 (CCNA2 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    L2V; Δ destruction box = deletion of aa47-55

    Promoter

    Available since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

    Plasmid

    #158604

    Purpose

    Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

    Plasmid

    #187994

    Purpose

    Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    mucin2 D1D2D'D3 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

    Plasmid

    #74932

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag-RNF168 delta MIU1/MIU2

    Plasmid

    #133981

    Purpose

    mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU1 and MIU2

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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