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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-38-HiBiT

Plasmid

#198315

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-38-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa39-331-HiBiT

Plasmid

#198316

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa39-331-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_aa1-76-HiBiT

Plasmid

#198314

Purpose

Loads a truncated payload; for identification of Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_aa1-76-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-SunTag

Plasmid

#198300

Purpose

Produces PVCs retargeted with SunTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-SunTag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGJJ055

Plasmid

#183622

Purpose

ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGC4780

Plasmid

#113408

Purpose

Translation reporter with inducible translation coupling (high native GFP expression)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

158_33121123_1

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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EZ-L498

Plasmid

#131771

Purpose

PPGK1_FUSN_Citrine _PixE_TCYC1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PPGK1_FUSN_Citrine _PixE_TCYC1

Use

Integration into his3 locus

Expression

Yeast

Promoter

PGK

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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68Q-ymsfGFP

Plasmid

#128560

Purpose

Httex1 (without proline-rich region) fused to ymsfGFP

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymsfGFP

Tags

ymsfGFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDiySpike2

Plasmid

#136949

Purpose

Plasmid for the In Vitro transcription of DiySpike2

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike2

Use

Synthetic Biology

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFS_0385_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1

Plasmid

#116997

Purpose

Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Rivularia sp. PCC 7116 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1G09

Plasmid

#70837

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

TfIIEalpha (TfIIEalpha Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
Rab36 cds in pGem-T Easy

Plasmid

#80557

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab36 (rab36 Zebrafish)

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_mTagBFP2

Plasmid

#250898

Purpose

mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PBAD_R1_EGFP

Plasmid

#250900

Purpose

EGFP expressed under araBAD promoter; part of BNeu AS13; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PBAD

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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