We narrowed to 9,174 results for: Pol

1. pKHH030_sgCtrl#1

   Plasmid

   #174142

   Purpose

   Lentiviral vector expressing a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mouse av full length

   Plasmid

   #217809

   Purpose

   Full length mouse integrin av expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGAV (Itgav Mouse)
   

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ACTB-(A)4x

   Plasmid

   #112058

   Purpose

   ACTB mRNA tagged with 4 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   4 copies of Riboglow tag after stop codon

   Promoter

   CMV

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3Ai-Cas9n-UGI-NLS

   Plasmid

   #109425

   Purpose

   Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNK7187

   Plasmid

   #219761

   Purpose

   MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PpASCL
   

   Expression

   Plant

   Promoter

   p35s_0.4kb - 5'UTR TMV omega

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT USP7 TRAF

   Plasmid

   #227226

   Purpose

   Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 62-205

   Promoter

   T7-promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1a-extGluc-mKate-NGFR

   Plasmid

   #104831

   Purpose

   extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   mKate
   NGFR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-N-Myc_884-1102 USP7

    Plasmid

    #131249

    Purpose

    Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTWIN1-His6-Ssp-Httex1-43Q

    Plasmid

    #84352

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid

    #127179

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mammalian

    Promoter

    TetO Promoter

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPRv2_sgCtrl#2

    Plasmid

    #174149

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-UbC-tdTomato

    Plasmid

    #62516

    Purpose

    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    tdTomato
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-N-Myc_775-1102 USP7

    Plasmid

    #131250

    Purpose

    Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-AMPH1-BAR-mCherry

    Plasmid

    #85130

    Purpose

    BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTon1(il11)

    Plasmid

    #233647

    Purpose

    Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

    Depositor

    Takeo Kubo

    Article

    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

    Inserts

    interleukin-11.L (350-913) (il11.L Frog)
    target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    

    Use

    Amphibian expression, tet-on

    Mutation

    Some synonymous substitutions were introduced in…

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse a2b full length

    Plasmid

    #217812

    Purpose

    Full length mouse integrin a2b expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA2B (Itga2b Mouse)
    

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACTB-(A)1x

    Plasmid

    #112055

    Purpose

    ACTB mRNA tagged with 1 copy of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    1 copy of Riboglow tag (variant A) after stop cod…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNK2657

    Plasmid

    #219741

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    hispidin- synthase from Mycena citricolor
    

    Use

    Synthetic Biology

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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