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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003_sgTFAP4 guide 1

Plasmid

#193450

Purpose

ko TFAP4 guide 1

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

Tfap4

Use

Lentiviral

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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MON

Plasmid

#187420

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-KOC207-MXMT

Plasmid

#182730

Purpose

Transient expression of KOC207-MXMT in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KOC207

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPEPT1

Plasmid

#195001

Purpose

Production of peptides in E. coli as GB1 fusion

Depositor

Marko Hyvönen

Article

Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

Type

Empty backbone

Tags

His8-tag and StrepTagII-GB1

Expression

Bacterial

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GB3270

Plasmid

#193114

Purpose

Random sequence R3 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R3

Use

Synthetic Biology

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GB3269

Plasmid

#193113

Purpose

Random sequence R2 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R2

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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GB2815

Plasmid

#193112

Purpose

Random sequence R1 of 1240 bp for A1 distal promoter position.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP (A1) Random Sequence R1

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-Dimer-1SUMO

Plasmid

#190249

Purpose

Expression dimer-1SUMO component in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

dimer-SUMO

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO7-6SIM

Plasmid

#190250

Purpose

Expression SUMO7-SIM6 in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

7SUMO-6SIM

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRS304-PTDH3-HEXAMER

Plasmid

#190251

Purpose

Expression hexamer component in yeast

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

hexamer

Tags

mCherry

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_AtU626Ter26

Plasmid

#191772

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_AtU626Ter26

Plasmid

#191775

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P2_AtU626Ter26

Plasmid

#191770

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_AtU626Ter26

Plasmid

#191773

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_AtU626Ter26

Plasmid

#191774

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID2S

Plasmid

#188647

Purpose

Expressing Target-AID2S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA

Plasmid

#164794

Purpose

pLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

4xMyc

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

Plasmid

#192113

Purpose

Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Insert

SARS-CoV-2-NTD-BA.4/5 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KEE

Plasmid

#187419

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SNG

Plasmid

#187424

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
OST

Plasmid

#187422

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TEG

Plasmid

#187421

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FER

Plasmid

#187418

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CHI

Plasmid

#187417

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1
ATP1B1

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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