We narrowed to 9,253 results for: CAG

1. REV human NANOS3 TALEN

   Plasmid

   #59723

   Purpose

   TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   NG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pJMP1221

   Plasmid

   #119260

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Carol Gross, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSUPER IKKalpha

   Plasmid

   #26208

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   pSUPER IKK alpha (CHUK Human)
   

   Use

   RNAi

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

4. GFP-GW182delta1

   Plasmid

   #11592

   Depositor

   Edward Chan

   Article

   Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

   Insert

   GW182 (TNRC6A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains only aa1-498; SEE DEPOSITOR NOTES BELOW

   Available Since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pAV115

   Plasmid

   #63182

   Purpose

   pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDA_834

   Plasmid

   #216087

   Purpose

   Cas9 [Sp] knockout targeting CD59, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD59 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX459-sg-mGreenLantern/EGFP

   Plasmid

   #179913

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern/EGFP sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pgRNA-non-target

   Plasmid

   #129465

   Purpose

   Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Non-target/random gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTX209

    Plasmid

    #89269

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01 and OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

    Plasmid

    #164040

    Purpose

    Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    sgTS5-sgTS6-sgTS7
    

    Use

    CRISPR

    Promoter

    mouse U6

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV105

    Plasmid

    #63195

    Purpose

    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRvCAHS3-mEGFP

    Plasmid

    #205008

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRvACT-mCherry

    Plasmid

    #205002

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP1170

    Plasmid

    #119252

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GG-EBNA-EEA-guide1-PGK-Puro

    Plasmid

    #102904

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV104

    Plasmid

    #63194

    Purpose

    pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAV123

    Plasmid

    #63189

    Purpose

    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGoldyTALEN-VIM-TAL-2

    Plasmid

    #62852

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge