We narrowed to 9,174 results for: Pol

1. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

   Plasmid

   #208944

   Purpose

   A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS2242

   Plasmid

   #111897

   Purpose

   ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple321 (TUBB3 Human)
   

   Use

   AAV

   Promoter

   Ple321 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-Med25-VBD-6His

   Plasmid

   #64773

   Purpose

   Bacterial expression of His-tagged Med25-VBD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VBD (MED25 Human)
   

   Expression

   Bacterial

   Mutation

   insert is 394-543 only

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. human a5 full length

   Plasmid

   #217818

   Purpose

   Full length human integrin a5 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGA5 (ITGA5 Human)
   

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKHH030_sgRB1#2

   Plasmid

   #174145

   Purpose

   Lentiviral vector expressing a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-Y986H

   Plasmid

   #174801

   Purpose

   Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Y986H (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y986H, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG416GAL-DENV_NS3-GS-NS2B

    Plasmid

    #201940

    Purpose

    Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    DENV NS3-GS-NS2B (POLY )
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2274

    Plasmid

    #111900

    Purpose

    ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple344 (TUBB3 Human)
    

    Use

    AAV

    Promoter

    Ple344 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid

    #193717

    Purpose

    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)
    

    Tags

    2xmNeongreen-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid

    #193720

    Purpose

    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTV001

    Plasmid

    #130265

    Purpose

    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA::His8
    

    Tags

    polyhistidine (internal)

    Expression

    Bacterial

    Mutation

    8His polyhistidine encoding region and XhoI site …

    Promoter

    araBAD

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACTB-(A)3x

    Plasmid

    #112057

    Purpose

    ACTB mRNA tagged with 3 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    3 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACTB-(A)2x

    Plasmid

    #112056

    Purpose

    ACTB mRNA tagged with 2 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    2 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-EglN2 H358A-pBabe-puro

    Plasmid

    #22705

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    Egg laying Nine - 2 (EglN2) (EGLN2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated cDNA: point mutation in codon 358 changi…

    Available Since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME mKate2-dynactin1-811

    Plasmid

    #105971

    Purpose

    entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    dynactin1-811 (DCTN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    contains only amino acids 1-811

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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