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YIp ADE2-LEU2

Plasmid

#122905

Purpose

ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

Plasmid

#106182

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

hSynapsin

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EB2316

Plasmid

#87753

Purpose

pkaiBC::EYFP cloned between XbaI and XhoI of EB2065

Depositor

Erin O'Shea

Publication

Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pkaiBC::EYFP

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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POT4-TDH3p-ycrtI-ADH1t

Plasmid

#65355

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtI-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFAB815

Plasmid

#47854

Purpose

BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rnpB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO9

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pLV.CMVenh.gp91.GP91.cHS4

Plasmid

#30479

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/GP91/cHS4

Use

Lentiviral

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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hSyn1-DIO-mCherry-CAAX

Plasmid

#223673

Purpose

Cre-dependently expresses mCherry-CAAX in neurons

Depositor

Jun Nagai

Publication

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV and Cre/Lox

Promoter

hSyn1

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_RSV

Plasmid

#175958

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

Depositor

Paul Freemont

Publication

Unruh et al medRxiv 2024.04.17.24305965

Inserts

P gene
M gene

Expression

Bacterial

Promoter

T7

Available since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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OmpX

Plasmid

#213136

Purpose

Expresses outer membrane protein X

Depositor

Sebastian Hiller

Publication

Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

Insert

OmpX (24-171) (ompX E.coli (K12))

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-WT-V5

Plasmid

#196018

Purpose

Expresses CRKL WT, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-biRhoBAST

Plasmid

#196344

Purpose

Overexpression of circular biRhoBAST

Depositor

Murat Sunbul

Publication

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biRhoBAST

Expression

Mammalian

Promoter

U6+27

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Publication

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

SV40

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-PtH2B

Plasmid

#133390

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

No promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CPspaH2B

Plasmid

#133385

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-HphMx

Plasmid

#122895

Purpose

ADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LRG/Foxa1 e2.4

Plasmid

#105511

Purpose

Lentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNA

Depositor

Christopher Vakoc

Publication

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (sgRNA, e2.4)

Use

Lentiviral

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pNR276

Plasmid

#79577

Purpose

TP901 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Publication

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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