-
-
EF1a_FOXA3_P2A_Hygro_Barcode
Plasmid#120440PurposeBarcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXA3 (FOXA3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11056
Plasmid#68258PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plantsDepositorInsertPromoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp13
Plasmid#157712Purposemammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LacI-11/109-pHG165c
Plasmid#90059PurposeDimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109DepositorInsertLacI-11/109
UseTagsExpressionBacterialMutation109PromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-TAF15N-mCherry-sspB
Plasmid#122436PurposeExpresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.DepositorInsertTAF15 (TAF15 Human)
UseLentiviralTagsmCherry and sspBExpressionMammalianMutationContains only amino acids 1-208PromoterSFFVAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-FLCN
Plasmid#72290PurposeoverexpressionDepositorInsertFLCN (FLCN Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag2AUF1(p45)
Plasmid#135947PurposeExpresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2ExpressionMammalianMutationPromoterCMV and SP6Available sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-FNIP2
Plasmid#72294PurposeoverexpressionDepositorInsertFNIP2 (FNIP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
psigma2-EGFP
Plasmid#53610PurposeExpresses sigma2 of adaptor protein of AP2DepositorInsertsigma2 (Ap2s1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_H2A_C1
Plasmid#85053PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
cyto-YFP-FKBPx5
Plasmid#103777Purposeexpression of iPOLYMER component in mammalian cells; has nuclear exclusion sequenceDepositorInsertcyto-YFP-FKBPx5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_FOXA1_P2A_Hygro_Barcode
Plasmid#120438PurposeBarcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXA1 (FOXA1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
INPP5D-eGFP WT
Plasmid#161998PurposeMammalian expression vector, containing a GFP tagged version of INPP5DDepositorInsertINPP5D (INPP5D Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-EGFP-hRhoA-wt
Plasmid#133926PurposeLow level expression of GFP fused human RhoA wild typeDepositorInsertGFP-hRhoA WT (RHOA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE6.3
Plasmid#102916Purposeexpresses ABE6.3 in mammalian cellsDepositorInsertABE6.3
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn1_UBC_Bla
Plasmid#61473Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PhLP1-myc-6xHis
Plasmid#218176PurposeExpresses phosducin-like protein 1 in mammalian cellsDepositorInsertPhosducin like protein 1 (PDCL Human)
UseTags6x His and MycExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-LSL-nlsGFP-shRNA
Plasmid#175584PurposeConditional express shRNA with control of LoxPDepositorTypeEmpty backboneUseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
