We narrowed to 10,454 results for: ESP

1. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRHDGAL43C

   Plasmid

   #218279

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

   Plasmid

   #207136

   Purpose

   DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP Cav2.2 D122A pcDNA3

   Plasmid

   #206068

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2trLATm2allL+A

   Plasmid

   #203750

   Purpose

   Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trLATm2(allL+A) (LAT Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_023d-sgCiPOLR2D

   Plasmid

   #202445

   Purpose

   Expression of a CRISPRi guide targeting POLR2D

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   POLR2D gRNA (POLR2D Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_003-sgUBR4

   Plasmid

   #202442

   Purpose

   Expression of a guide RNA targeting UBR4

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   UBR4 gRNA (UBR4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_003-sgUBA6

   Plasmid

   #202440

   Purpose

   Expression of a guide RNA targeting UBA6

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   UBA6 gRNA (UBA6 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2478

    Plasmid

    #142101

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid

    #201024

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX459_GRHL1-exon5

    Plasmid

    #177763

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX459_GRHL1-exon8

    Plasmid

    #177764

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

    Plasmid

    #177767

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Expression

    Mammalian

    Mutation

    R9A

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

    Plasmid

    #177768

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Expression

    Mammalian

    Mutation

    S95A

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

    Plasmid

    #177769

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Expression

    Mammalian

    Mutation

    K116A

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

    Plasmid

    #177770

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Expression

    Mammalian

    Mutation

    T139A

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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