We narrowed to 9,174 results for: Pol

1. pCAG-Flag-Avi-ires-CHD5

   Plasmid

   #68878

   Purpose

   Transient mammalian expression of CHD5 for BirA tagging

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   FLAG/AVI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTWIN1-His6-Ssp-Httex1-29Q

   Plasmid

   #84350

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_6xHis-PARP1-A774L

   Plasmid

   #174796

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A774L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A774L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_6xHis-PARP1-W318R

   Plasmid

   #174793

   Purpose

   Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-W318R (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W318, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTWIN1-His6-Ssp-Httex1-37Q

   Plasmid

   #84351

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUDP044

   Plasmid

   #101168

   Purpose

   pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTWIN1-His6-Ssp-Httex1-15Q

   Plasmid

   #84348

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3 Flag MFF iso5 S172A

   Plasmid

   #74382

   Purpose

   Flag tagged human S172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3 Flag MFF iso5 S155,172A

   Plasmid

   #74383

   Purpose

   Flag tagged human S155,172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   See 155 Ala, Ser 172 Ala (numbering based on MFF …

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pEMS2272

    Plasmid

    #111898

    Purpose

    ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple342 (TUBB3 Human)
    

    Use

    AAV

    Promoter

    Ple342 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-GST-CHD5(Chromos)-FRAG

    Plasmid

    #68875

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only the two chromodomains of CHD5

    Promoter

    tac

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Cherry-SRBC

    Plasmid

    #69492

    Purpose

    Expression of fluorescently tagged SRBC in mammalian cells

    Depositor

    Rob Parton

    Article

    Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

    Insert

    SRBC (Cavin3 Mouse)
    

    Tags

    Cherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 Flag MFF iso5 S155A

    Plasmid

    #74381

    Purpose

    Flag tagged human S155A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. CMV-10-3xFLAG-LGP2-K651E

    Plasmid

    #58685

    Purpose

    Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

    Insert

    LGP2-K651E (DHX58 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    mutation to CTD, K651E

    Promoter

    CMV

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-FLAG-CHD5-WT-331-672aa

    Plasmid

    #68870

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa)

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa

    Promoter

    CMV

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEMS1136

    Plasmid

    #29056

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple198 (SLC6A4 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn1-DIO-eGFP

    Plasmid

    #227237

    Purpose

    Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

    Depositor

    Richard Huganir

    Article

    Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

    Insert

    eGFP (eGFP )
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge