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pcDNA5 FRT TO myc Separase delta 55/S1126A

Plasmid

#59825

Purpose

Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55/S1126A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL G457D

Plasmid

#61367

Purpose

Expresses Human full length RIPK3 with G457Dmutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with G457D mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed G457 to D

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL N464D

Plasmid

#61369

Purpose

Expresses Human full length RIPK3 with N464D mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with N464D mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed N464to D

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/MPL-V5

Plasmid

#214794

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL (MPL Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR del52-His

Plasmid

#214795

Purpose

Baculovirus expression of human CALR del52-His

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52-His (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR ins5-His

Plasmid

#214796

Purpose

Baculovirus expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wt

Plasmid

#214797

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wt (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR del52

Plasmid

#214798

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5

Plasmid

#214799

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL(N117Q)

Plasmid

#214800

Purpose

Mammalian expression of human MPL(N117Q)

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL(N117Q) (MPL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL(N178Q)

Plasmid

#214801

Purpose

Mammalian expression of human MPL(N178Q)

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL(N178Q) (MPL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL(N298Q)

Plasmid

#214802

Purpose

Mammalian expression of human MPL(N298Q)

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL(N298Q) (MPL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL(N358Q)

Plasmid

#214803

Purpose

Mammalian expression of human MPL(N358Q)

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL(N358Q) (MPL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR WTΔKDEL

Plasmid

#214790

Purpose

Mammalian expression of human CALR wtΔKDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wtΔKDEL (CALR Human)

Tags

non

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wtΔKDEL

Plasmid

#214791

Purpose

Mammalian expression of human CALR wtΔKDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wtΔKDEL (CALR Human)

Use

Retroviral

Tags

non

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-DsRed/MPL

Plasmid

#214793

Purpose

Mammalian expression of human MPL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human MPL (MPL Human)

Use

Retroviral

Tags

non

Promoter

MSCV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_delta_EHMT1

Plasmid

#228816

Purpose

Expression of human EHMT1 with deletion of region between N-terminal 300 aa and C-terminal Ank+SET

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of region between N-terminal 300 aa and …

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9A(E195Q)-HA

Plasmid

#209256

Purpose

Mammalian expression of ATP9A mutant E195Q

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9A (ATP9A Human)

Use

Retroviral

Tags

HA

Mutation

E195Q

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9B(E272Q)-HA

Plasmid

#209257

Purpose

Mammalian expression of ATP9B mutant E272Q

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9B (ATP9B Human)

Use

Retroviral

Tags

HA

Mutation

E272Q

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-WzzE

Plasmid

#196661

Purpose

Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Tags

TEV-cleavable His6

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-WzzE

Plasmid

#196662

Purpose

Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Tags

TEV-cleavable GST

Expression

Bacterial

Mutation

Valine insertion at position 2.

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-mCherry-WzzE

Plasmid

#196663

Purpose

Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Tags

His and mCherry

Expression

Bacterial

Mutation

Valine insertion at position 2 of WzzE.

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mCherry

Plasmid

#196664

Purpose

Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Tags

TEV-cleavable distrep tag II and mCherry

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPglL-strep-mClover3

Plasmid

#196665

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-GST-distrepII

Plasmid

#196666

Purpose

Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

GST and TEV-cleavable di-strep tag II

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-strep-mClover3

Plasmid

#196668

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C39A-Myc

Plasmid

#201167

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A

Promoter

MESV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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p426-Cup1p-FusionRed

Plasmid

#188392

Purpose

Cu2+-dependent expression of red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FusionRed

Expression

Yeast

Promoter

CUP1 promoter

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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