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Showing: 1601 - 1650 of 8299 results

  21. pCK01_PalkS_alkS

    Plasmid
    #166502
    Purpose
    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.
    Depositor
    Sven Panke
    Article
    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2
    Insert
    alkS
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    L401G
    Promoter
    PalkS
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166502-plasmid-map-sequence-id-333027

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  23. pTrex-n-eGFP

    Plasmid
    #62544
    Purpose
    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome
    Depositor
    Rick Tarleton
    Article
    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.
    Insert
    eGFP
    Use
    (1)trypanosoma cruzi expression of egfp (2) genomā€¦
    Tags
    None
    Expression
    Mutation
    None
    Promoter
    Ribo-HX1
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62544-plasmid-map-sequence-id-109759

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  31. pRPa-iSL(MCS1+2)

    Plasmid
    #69244
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69244-plasmid-map-sequence-id-124145

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  33. pSB1K3_pBen-sfGFP_J23101-BenR

    Plasmid
    #128125
    Purpose
    Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032
    Depositor
    Jean-Loup Faulon
    Article
    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:
    Insert
    sfGFP and BenR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBen and J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128125-plasmid-map-sequence-id-252642

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  34. pNCMV 16E6no* delPDZ

    Plasmid
    #47678
    Purpose
    PDZ-binding motif is deleted
    Depositor
    Karl Munger
    Article
    Spangle et al J Virol. 2012 May 2.
    Insert
    HPV 16 E6no* (E6 Human, HPV)
    Use
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    del first 7aa and last 6aa, V42L*
    Promoter
    CMV
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pCS2-EcYRS-EGFP*

    Plasmid
    #191118
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Use
    Tags
    His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191118-plasmid-map-sequence-id-373723

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  43. BPTI-21

    Plasmid
    #195058
    Purpose
    simplyfied amino acid sequence by alanine scanning
    Depositor
    Yutaka Kuroda, Peter Kim
    Article
    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d
    Insert
    bovine pancreatic trypsin inhibitor
    Use
    Tags
    His tag and trpĪ”LE leader sequences
    Expression
    Bacterial
    Mutation
    21 amino acids are Alanine out of 58 amino acids
    Promoter
    T7 promotor
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195058-plasmid-map-sequence-id-388061

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  44. pBAD-MtrxRFP2

    Plasmid
    #172323
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172323-plasmid-map-sequence-id-431098

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  47. pBEAST_J23101-HipO

    Plasmid
    #128133
    Purpose
    The cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032
    Depositor
    Jean-Loup Faulon
    Article
    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:
    Insert
    HipO
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128133-plasmid-map-sequence-id-252651

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  49. pRPa-c6MYCn

    Plasmid
    #69242
    Purpose
    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69242-plasmid-map-sequence-id-123680

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  50. pRP-c6MYCn

    Plasmid
    #69243
    Purpose
    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Expression
    Mutation
    Promoter
    RRNA promoter
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69243-plasmid-map-sequence-id-123691

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Showing: 1601 - 1650 of 8299 results