We narrowed to 7,050 results for: ef1a
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Plasmid#124604PurposeCre-dependent soma-localized C1V1(t/t) for optogenetic stimulationDepositorInsertSoma-localized C1V1(t/t)
UseAAVTagsmRuby2ExpressionMammalianMutationPromoterEf1aAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233443PurposePiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)DepositorInsertFKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceJune 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP
Plasmid#233446PurposePiggyBac donor plasmid to overexpress ADAR2dd-PCPDepositorInsertADAR2dd-PCP-T2A-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0250 (PB)EF1a-CCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233441PurposePiggyBac donor plasmid to overexpress CCR6-ADAR2dd (gLIDAR)DepositorInsertCCR6V2-ADAR2dd-IRESv0-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP
Plasmid#233444PurposePiggyBac donor plasmid to overexpress ADAR2dd-MCPDepositorInsertADAR2dd-MCP-T2A-PuroR-P2A-BFP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233046PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA
Plasmid#233045PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.DepositorInsertRfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export
Plasmid#233382PurposeOptimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR1(ecto+TM)-ADAR2dd
UseTagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export
Plasmid#233386PurposeOptimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertIL2Rb(ecto+TM)-ADAR2dd
UseTagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export
Plasmid#233389PurposeOptimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequenceDepositorInsertIL2Rg(ecto+TM)-tdMCP
UseTagsHAExpressionMammalianMutationWTPromoterEF1aAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLAG-mouse Beta Adrenergic Receptor (#1)
Plasmid#233041PurposeTo Express a CRE dependent Flag tagged Mouse Beta Adrenergic Receptor from a EF1a promoterDepositorInsertmouse Beta Adrenergic Receptor (Adrb1 Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)
Plasmid#229742PurposeExpresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
UseTagsHaloExpressionMammalianMutationLIR1 mutant WEVL -> AEVAPromoterEF1AAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)
Plasmid#229743PurposeExpresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
UseTagsHaloExpressionMammalianMutationLIR2 mutant WTFI -> ATFAPromoterEF1AAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
Plasmid#220613PurposeCre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.DepositorInsertFLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
UseAAV and Cre/LoxTagsFLAG; ArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet
Plasmid#220614PurposeCre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.DepositorInsert3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
UseAAV and Cre/LoxTags3x HA; ArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH
Plasmid#131683PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1DepositorInsertsEGFP-KASH
SpCas9 sgRNA vs mouse GRID1
UseAAVTagsKASHExpressionMutationPromoterEF1a and mU6Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
Plasmid#184636PurposeExpresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVTagsExpressionMutationGat1 gene with Arginine 69 changed to Lysine, to …PromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)
Plasmid#171100PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.DepositorInserthSpCas9 (Neurog2 Mouse, S. pyogenes)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)
Plasmid#171099PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.DepositorInserthSpCas9 (Ascl1 Mouse, S. pyogenes)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118159PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3
Plasmid#118160PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)
Plasmid#217306PurposesgRNA mcherry + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
UseCRISPRTagsExpressionMutationPromoterU6, Ef1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP
Plasmid#31481DepositorInsertTraffic Light Reporter 1.1 (Sce target) EF BFP
UseLentiviralTagsExpressionMutationmCherry contains M9S and M16L to reduce backgroun…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
Plasmid#31482DepositorInsertTraffic Light Reporter 1.1 (Sce Target) EF Puro
UseLentiviralTagsExpressionMutationmCherry has M9S and M16L mutations. This is what …PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR
Plasmid#210523PurposeAAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsGFP-KASH
U6-gRNA-directcapture
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR
Plasmid#213480PurposeAAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.DepositorInsertsmtagBFP-KASH
U6-gRNA-directcapture
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Plasmid#20298PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsEYFPExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA
Plasmid#166693PurposeExpress dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.DepositorInsertsdCas9-VPR
3x Cnga1 promoter-targeting sgRNAs
UseCRISPRTagsExpressionMammalianMutationPromoterTRE and U6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Plasmid#20297PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR
Plasmid#154827PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)DepositorInsertsTagBFP
HygR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianMutationPromoterCMV, EF1a, and SV40 early promoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro
Plasmid#141371PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationC145A, human codon optimizedPromoterEF1alphaAvailable sinceApril 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
Plasmid#192822PurposeGolden Gate compatible plasmid with mCherry and NeoDepositorInsertmCherry and Neo
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP
Plasmid#232800PurposeHelper plasmid to trigger auxin-inducible-degradation of targeted proteins.DepositorInsertOsTIR1 F74G EGFP-AID
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR
Plasmid#154826PurposeLanding pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)DepositorInsertsmCherry
NeoR
ZsGreen1-DR
UseCRISPR and Cre/LoxTagsPESTExpressionMammalianMutationPromoterCMV, EF1a, and SV40 early promoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188773PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
Plasmid#31483DepositorInsertTraffic Light Reporter 2.1 (V2468 zfn target) EF Puro
UseLentiviralTagsExpressionMutationTraffic Light 2.0 series have a +3 PEST sequence …PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)
Plasmid#229839PurposeA CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.DepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167971PurposeTp63 reporter targeting repair templateDepositorInsertTp63 (TP63 Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template
Plasmid#167943PurposeSFTPC reporter targeting repair templateDepositorInsertSFTPC (SFTPC Human)
UseCRISPR; Donor templateTagsVenusExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterEF1aAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)
Plasmid#217305PurposesgRNA BFP + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-BFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6, Ef1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140229PurposeDox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFPDepositorInsertAntiCRISPR protein AcrVA1
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
Plasmid#31480DepositorInsertTraffic Light Reporter 1.1 (ani target)
UseLentiviralTagsExpressionMutationmCherry has M9S, M16L to reduce background mCherr…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)
Plasmid#207357PurposeLentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP
Plasmid#233418PurposePiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRESDepositorInsertCCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceJune 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA
Plasmid#235325PurposePiggyBac integration vector for ComMAND base gene regulating mRuby2DepositorInsertmRuby2
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
Plasmid#233435PurposePiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRESDepositorInsertCCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
Plasmid#233426PurposePiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRESDepositorInsertAVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)
Plasmid#207356PurposeLentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)
Plasmid#176139PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only