Addgene

We narrowed to 2,364 results for: buc

Showing: 641 - 660 of 2364 results

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M-ST1-VPR

Plasmid

#68498

Purpose

nuclease competent ST1-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

ST-Cas9-VPR

Use

Tags

VPR

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pMpGWB106-OEP7(1–50)

Plasmid

#100602

Purpose

Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50)-Citrine (OEP7 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV-ArchT-GFP

Plasmid

#86536

Purpose

Retrovirus expressing optogenetic silencer ArchT-GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

ArchT

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Synapsin I (human)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Rhoc-wt_3'UTR

Plasmid

#31884

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc wild-type 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pCINTBeta1

Plasmid

#14067

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

PstI-BglI fragment was blunt end cloned and EcoRI…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C373T

Plasmid

#24734

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced by T at position 373 resulting in Q125…

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C1271T

Plasmid

#24736

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced with T at position 1271 resulting in A…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
1010Av2

Plasmid

#125642

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Use

Tags

Expression

Insect

Mutation

Promoter

10xUAS

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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1010Bv2

Plasmid

#125643

Purpose

Expresses FAR protein under 10xUAS control

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

FAR protein

Use

Tags

Expression

Insect

Mutation

Promoter

10xUAS

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-BSD

Plasmid

#234071

Purpose

For lentiviral expression of PEmax with blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

EF-1α core promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

AVI tag and His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-c-HA

Plasmid

#209239

Purpose

Mammalian expression of ATP11C isoform c

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform c (ATP11C Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-b-HA

Plasmid

#209238

Purpose

Mammalian expression of ATP11C isoform b

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207_K

Plasmid

#146755

Purpose

Bacterial Expression of HsSmg6_1-207

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207 (SMG6 Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12-EBMmut_K

Plasmid

#146767

Purpose

Mammalian Expression of HsRRP12-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12-EBMmut (RRP12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg6_577-816-siRNAres_I

Plasmid

#146559

Purpose

Mammalian Expression of HsSmg6_577-816-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_577-816-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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