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Plasmid#118162PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118161PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2
Plasmid#118163PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3
Plasmid#118164PurposeCRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDSDepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
SbMBP
Plasmid#117861Purposeprotein expressionDepositorInsertSbMBP
UseTagsExpressionYeastMutationPromoterAOXAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD5_w
Plasmid#112645PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.DepositorInsertU6:3-tRNA-sgRNA expression cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
C5m
Plasmid#120957PurposeMoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).DepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9fl
Plasmid#228409PurposeExpression of NIT-9 in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
TU#707
Plasmid#15276DepositorInsertnzgfp
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
TU#711
Plasmid#15279DepositorInsertczgfp
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
TU#710
Plasmid#15278DepositorInsertnzgfp
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
TU#713
Plasmid#15281DepositorInsertczyfp
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
TU#714
Plasmid#15282DepositorInsertnzcfp
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
TU#715
Plasmid#15283DepositorInsertczcfp
UseTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9E
Plasmid#228419PurposeExpression of NIT-9E domain in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of the N terminal G domain and linkageā¦PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9G
Plasmid#228420PurposeExpression of NIT-9G domain in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of the C terminal E domain and linkageā¦PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_A184-G203
Plasmid#228421PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of A184-G203PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_G204-G223
Plasmid#228422PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of G204-G223PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_E224-G243
Plasmid#228423PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of E224-G243PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_K264-P282
Plasmid#228425PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of K264-P282PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only