Skip to main content
Addgene
Showing: 641 - 660 of 5270 results
  1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118161
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. SbMBP

    Plasmid
    #117861
    Purpose
    protein expression
    Depositor
    Insert
    SbMBP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Dec. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. C5m

    Plasmid
    #120957
    Purpose
    MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).
    Depositor
    Insert
    mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pCCG_nit9fl

    Plasmid
    #228409
    Purpose
    Expression of NIT-9 in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pCCG_nit9E

    Plasmid
    #228419
    Purpose
    Expression of NIT-9E domain in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the N terminal G domain and linkage…
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pCCG_nit9G

    Plasmid
    #228420
    Purpose
    Expression of NIT-9G domain in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the C terminal E domain and linkage…
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pCCG_nit9_A184-G203

    Plasmid
    #228421
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of A184-G203
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pCCG_nit9_G204-G223

    Plasmid
    #228422
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of G204-G223
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pCCG_nit9_E224-G243

    Plasmid
    #228423
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of E224-G243
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pCCG_nit9_K264-P282

    Plasmid
    #228425
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of K264-P282
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 641 - 660 of 5270 results