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Kv2.1 K+ channel scFv [K39/25]

Plasmid

#182067

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [K89/34]

Plasmid

#182069

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K89/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pVH19-Tier1-PhCMV-p300core

Plasmid

#169572

Purpose

Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P300core (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

Plasmid

#132560

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop replaced by com aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-2xMS2

Plasmid

#132554

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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hHSF1 S307A

Plasmid

#118364

Purpose

This plasmid expresses human HSF1 S307A mutant.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S307A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag

Plasmid

#154970

Purpose

Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

insert codes for EBA175 residues 1284 to 1462

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Nav1.7 Na+ channel scFv [N68/6]

Plasmid

#182094

Purpose

Mammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Aldh1L1 scFv [N103/31]

Plasmid

#182080

Purpose

Mammalian Expression of Aldh1L1 scFv. Derived from hybridoma N103/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Aldh1L1 (Rattus norvegicus) (Aldh1l1 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/43]

Plasmid

#182066

Purpose

Mammalian Expression of PSD-95 scFv. Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting PSD-95 (Homo sapiens) (DLG4 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Cav1.2 Ca2+ channel scFv [N263/31]

Plasmid

#182083

Purpose

Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#182078

Purpose

Mammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GluN2B/NR2B glutamate receptor scFv [N59/36]

Plasmid

#182093

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#182092

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CASPR/Neurexin IV scFv [K65/35]

Plasmid

#182068

Purpose

Mammalian Expression of CASPR/Neurexin IV scFv. Derived from hybridoma K65/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting CASPR/Neurexin IV (Rattus norvegicus) (Cntnap1 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Calretinin scFv [L122/68]

Plasmid

#182077

Purpose

Mammalian Expression of Calretinin scFv. Derived from hybridoma L122/68.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calretinin (Homo sapiens) (CALB2 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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Kv1.1 K+ channel scFv [K20/78]

Plasmid

#182065

Purpose

Mammalian Expression of Kv1.1 K+ channel scFv. Derived from hybridoma K20/78.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv1.1 K+ channel (Rattus norvegicus) (Kcna1 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Arl13b scFv [N295B/66]

Plasmid

#182086

Purpose

Mammalian Expression of Arl13b scFv. Derived from hybridoma N295B/66.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Arl13b (Mus musculus) (Arl13b Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RGS14 scFv [N133/21]

Plasmid

#182081

Purpose

Mammalian Expression of RGS14 scFv. Derived from hybridoma N133/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting RGS14 (Rattus norvegicus) (Rgs14 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge