Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 29,859 results for: REP

Showing: 9871 - 9900 of 29859 results

  4. LSB-hsa-miR-19a-3p

    Plasmid
    #103323
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-19a-3p target (MIR19A Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103323-plasmid-map-sequence-id-195695

    Enlarge

  6. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid
    #110624
    Purpose
    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    LP1B and hU6
    Available Since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110624-plasmid-map-sequence-id-211314

    Enlarge

  12. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

    Enlarge

  15. LSB-hsa-miR-29a-3p

    Plasmid
    #103391
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-29a-3p target (MIR29A Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103391-plasmid-map-sequence-id-195831

    Enlarge

  16. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

    Enlarge

  18. LSB-hsa-miR-29a-5p

    Plasmid
    #103392
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-29a-5p target (MIR29A Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    103392-plasmid-map-sequence-id-195833

    Enlarge

  22. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

    Enlarge

  23. LSB-hsa-miR-331-3p

    Plasmid
    #103444
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-331-3p target (MIR331 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103444-plasmid-map-sequence-id-195937

    Enlarge

  24. LSB-hsa-miR-485-3p

    Plasmid
    #103553
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-485-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-485-3p target (MIR485 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103553-plasmid-map-sequence-id-196154

    Enlarge

  26. pMXs-LB2O

    Plasmid
    #188036
    Purpose
    Fusion of human MYC Transactivation Domain (TAD) MYC Box II (MBII) with human OCT4 protein expressed in pMXs vector
    Depositor
    Young Tang
    Article
    Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1
    Insert
    MYC TAD MBII region fused with OCT4 (POU5F1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188036-plasmid-map-sequence-id-374827

    Enlarge

  27. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid
    #101231
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101231-plasmid-map-sequence-id-197986

    Enlarge

  28. LSB-hsa-miR-148a-3p

    Plasmid
    #103250
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-148a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-148a-3p target (MIR148A Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103250-plasmid-map-sequence-id-195549

    Enlarge

  30. pBGE1.0

    Plasmid
    #165986
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    165986-plasmid-map-sequence-id-375342

    Enlarge

Showing: 9871 - 9900 of 29859 results