We narrowed to 8,387 results for: BLI

1. pT5T_Rev7_1-211_SA112_KVK_YAK

   Plasmid

   #105630

   Purpose

   Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3

   Depositor

   Thanos Halazonetis

   Insert

   Rev7 (Mad2l2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…

   Promoter

   T7

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRESN2_GFP_YTA_Champ1_328-354

   Plasmid

   #105639

   Purpose

   Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   GFP YTA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MS2_SC2_S_B.1.640

   Plasmid

   #180228

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   B.1.640 (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Jan. 28, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. CD44U-c010

   Plasmid

   #175453

   Purpose

   Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MS2_SC2_S_C.1.2

   Plasmid

   #177367

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C1.2 (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Jan. 20, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. MS2_SC2_S_Mu

   Plasmid

   #177366

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mu (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Jan. 20, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. MS2_SC2_S_Kappa

   Plasmid

   #175953

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Kappa (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. MS2_SC2_S_Eta

   Plasmid

   #175954

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Eta (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. MS2_SC2_S_Gamma

   Plasmid

   #175047

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gamma (Amino Acid 319-869)

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. MS2_SC2_S_Theta

    Plasmid

    #175951

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Theta (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. MS2_SC2_S_Zeta

    Plasmid

    #175952

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Zeta (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pChd4EosHaloTAF_Puro

    Plasmid

    #172099

    Purpose

    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    chromodomain helicase DNA binding protein 4 (Chd4 Synthetic, Mouse)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid

    #174138

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid

    #174139

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172480

    Purpose

    H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    H2B-sfGFP-T2A-tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB6-Id1-T2A-tdTomato-FNF-TK

    Plasmid

    #139281

    Purpose

    B6J Id1-T2A-tdTomato allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid

    #140271

    Purpose

    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Gfap (Gfap Mouse)
    

    Use

    Mouse Targeting

    Tags

    C-terminal fusion of T2A-tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid

    #137044

    Purpose

    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Gfap (Gfap Mouse)
    

    Use

    Mouse Targeting

    Tags

    T2A-td-sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFE-sfGFP

    Plasmid

    #132998

    Purpose

    pFE carrying a sfGFP gene

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    sfGFP
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFE-dabA2

    Plasmid

    #133001

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    dabA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetR

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge