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PDPK1 gRNA (BRDN0001162582)

Plasmid

#77450

Purpose

3rd generation lentiviral gRNA plasmid targeting human PDPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PDPK1 (PDPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001145450)

Plasmid

#75572

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001147308)

Plasmid

#75574

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TJP2 gRNA (BRDN0001147569)

Plasmid

#77827

Purpose

3rd generation lentiviral gRNA plasmid targeting human TJP2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TJP2 (TJP2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TJP2 gRNA (BRDN0001148019)

Plasmid

#77828

Purpose

3rd generation lentiviral gRNA plasmid targeting human TJP2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TJP2 (TJP2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TJP2 gRNA (BRDN0001145932)

Plasmid

#77829

Purpose

3rd generation lentiviral gRNA plasmid targeting human TJP2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TJP2 (TJP2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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TP53RK gRNA (BRDN0001146381)

Plasmid

#77701

Purpose

3rd generation lentiviral gRNA plasmid targeting human TP53RK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TP53RK (TP53RK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TLK1_pLX307

Plasmid

#98378

Purpose

Lentiviral expression of TLK1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TLK1 (TLK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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SIN-CMV-Ca9-WPRE

Plasmid

#87874

Purpose

Transfer plasmid for the production of lentiviral vectors

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway entry vector

Promoter

H1, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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VRK1 gRNA (BRDN0001149172)

Plasmid

#75525

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK1 (VRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK1 gRNA (BRDN0001149207)

Plasmid

#75524

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK1 (VRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-ACTB-iTagRFP-T

Plasmid

#115916

Purpose

donor to insert iTagRFP-T at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-TagRFP-T

Use

Donor template

Tags

TagRFP-T

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149383)

Plasmid

#80238

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM7 gRNA (BRDN0001146152)

Plasmid

#76111

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001148546)

Plasmid

#76386

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001148298)

Plasmid

#76387

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE

Plasmid

#87885

Purpose

To produce lentiviral vector for HTT editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

shHTT1, cherry

Use

Lentiviral

Promoter

H1, PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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RIPK3 gRNA (BRDN0001147505)

Plasmid

#76996

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK3 (RIPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RIPK3 gRNA (BRDN0001145972)

Plasmid

#76997

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK3 (RIPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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