We narrowed to 8,324 results for: gnal

1. pCMV-NES-MBP-cATOM(Ras)

   Plasmid

   #209711

   Purpose

   Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   NES-MBP-cATOM(Ras)
   

   Use

   Tags

   NES-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TN-XXL pRSETB

   Plasmid

   #45796

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

   Insert

   TN-XXL
   

   Use

   Tags

   6xHIS, ECFP, and YFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. 3xFNLDD/pMXs-IG

   Plasmid

   #48972

   Purpose

   Expresses 3xFNLDD in mammalian cells

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

   Insert

   3xFNLDD
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBH31(gfcD)

   Plasmid

   #176199

   Purpose

   Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Use

   Tags

   pelC leader-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBH31(gfcD-10xHis)

   Plasmid

   #176200

   Purpose

   Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Use

   Tags

   pelC leader-10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIP1::eYFP

   Plasmid

   #135231

   Purpose

   UPR signaling reporter (upregulates eYFP expression under UPR conditions)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   pBiP1
   

   Use

   Tags

   eYFP

   Expression

   Plant

   Mutation

   Promoter

   BiP1

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFNLDD/pCMV-7.1

   Plasmid

   #48874

   Purpose

   Expresses 3xFNLDD in mammalian cells

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

   Insert

   3xFNLDD
   

   Use

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_ss-SBP-EGFP

   Plasmid

   #172358

   Purpose

   Reporter to monitoring a streptavidin-binding peptide trafficking

   Depositor

   Guido Kroemer

   Article

   Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

   Insert

   ER importing signal sequence
   

   Use

   Lentiviral

   Tags

   SBP-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Myog'-2A-mCherryNLS-PGK-Purodtk

   Plasmid

   #69547

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm (Myog Mouse)
   Myogenin 3' homology arm (Myog Mouse)
   Puro-delta-tk
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Expression

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Promoter

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NES-ZapCmR1.1

    Plasmid

    #59014

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

11. EC0_2_VP64

    Plasmid

    #163714

    Purpose

    Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    VP64 + SV40 NLS
    

    Use

    Synthetic Biology; Moclo vector level 0 (type 4a)

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Split-RESCUE-C

    Plasmid

    #170154

    Purpose

    AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    RESCUE-DDC-λN-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest_pK7WG2_pRPS5A_CTP OleGFP

    Plasmid

    #171723

    Purpose

    ptpTALECD vector assembly, Destination vector;

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. E2_pENTR_R4R3_T CTP

    Plasmid

    #171736

    Purpose

    ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGK415-­phlTA2-­1E

    Plasmid

    #165970

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCNL-N1_PTS2

    Plasmid

    #65676

    Purpose

    Expresses PTS2-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    peroxisomal targeting signal 2
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. EPHA7 (3NRU)

    Plasmid

    #28126

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPHA7:SDC173-D03:C209131 (EPHA7 Human)
    

    Use

    Tags

    His and Honeybee melittin Signal sequence (MKFLVN…

    Expression

    Bacterial

    Mutation

    contains amino acids residues 32-204.

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pCoofy62

    Plasmid

    #122014

    Purpose

    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-(RTRG)6

    Plasmid

    #69849

    Purpose

    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

    Insert

    6 RTRG repeats
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-λN-βLac

    Plasmid

    #162039

    Purpose

    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

    Depositor

    Karen Polizzi

    Article

    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

    Insert

    λN domain of the λ phage and a β-lactamase fusion protein
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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