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pKHH030_sgRB1#2

Plasmid

#174145

Purpose

Lentiviral vector expressing a sgRNA against the human RB1 gene

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

RB1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Expression

Mammalian

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0710

Plasmid

#133517

Purpose

Expression of mCherry tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0770

Plasmid

#133518

Purpose

Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E2 (20–629)

Plasmid

#132627

Purpose

expresses human LRP6 E1E2 (20–629) in Hi5 insect cell

Depositor

Wenqing Xu

Publication

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–629)

Expression

Insect

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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HT-ELK1

Plasmid

#33258

Depositor

Frederick Stanley

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ELK1 (ELK1 Human)

Tags

Halo Tag

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
DVK_AF

Plasmid

#66068

Purpose

MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only
pFastBac1-Ac-AChBP

Plasmid

#63128

Purpose

Expression of Aplysia californica AChBP

Depositor

Titia Sixma

Publication

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-LC-Myosin-N-7

Plasmid

#57396

Purpose

Localization: Light Chain Myosin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LC-Myosin

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pVRb22_up1147

Plasmid

#49716

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf22_up1147

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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ZO-2 shRNA 2434

Plasmid

#37211

Depositor

James Anderson, Alan Fanning

Publication

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-2 shRNA (TJP2 canine)

Use

RNAi

Expression

Mammalian

Available since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
ZO-2 shRNA 1320

Plasmid

#37210

Depositor

James Anderson, Alan Fanning

Publication

Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

Insert

dog ZO-2 shRNA (TJP2 canine)

Use

RNAi

Expression

Mammalian

Available since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pEF 3-GUS-2

Plasmid

#20104

Depositor

Antonio Richart

Insert

GUS (gus )

Use

Synthetic Biology; Plant gateway vector

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p2HGPD/ER/cyc

Plasmid

#1283

Depositor

Susan Lindquist

Publication

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

ER

Tags

Gpd promoter

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
EML6428_ pRL662-eGFP

Plasmid

#234350

Purpose

Expressed eGFP(S65T) in agrobacteria for tracing in planta

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Bacterial

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Publication

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Expression

Bacterial

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_122

Plasmid

#208095

Purpose

Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

Depositor

John Doench

Publication

Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSA12

Plasmid

#186546

Purpose

LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSA12

Tags

6xHis

Expression

Bacterial

Mutation

hfYFP-G65S/Y203I/Q204E/E222D/R223F

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD_Ub11TCA

Plasmid

#174517

Purpose

Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

Depositor

Jason W Chin

Publication

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

Ubiquitin

Tags

6xHis

Expression

Bacterial

Mutation

Recoded with TCA replacing codon 11 of wt ubiquit…

Promoter

araBAD

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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