We narrowed to 15,599 results for: LEA

1. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PET-B-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207378

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PET-Blackjack SpCas9-NLS-6xHis

   Plasmid

   #207375

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Blackjack SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST17-Ctdnep1_C-ter

   Plasmid

   #196525

   Purpose

   Expression of Ctdnep1_C-ter

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1-C-ter (CTDNEP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   No transmembrane domain

   Promoter

   T7 promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

   Plasmid

   #196877

   Purpose

   Neuron-specific expression of mOrange2 reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBactin-td-mCherry-CI

   Plasmid

   #196851

   Purpose

   Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eZiPro W5F

   Plasmid

   #198436

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Ute Hellmich, Tanja Schirmeister

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W5F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eZiPro W83F

   Plasmid

   #198439

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Ute Hellmich, Tanja Schirmeister

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W83F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eZiPro W69F

   Plasmid

   #198438

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Ute Hellmich, Tanja Schirmeister

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W69F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eZiPro W89F

    Plasmid

    #198440

    Purpose

    Activity/inhibition assays, 19F-NMR assignment

    Depositor

    Ute Hellmich, Tanja Schirmeister

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    enzymatically cleavable linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    W89F

    Promoter

    T7 promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eZiPro

    Plasmid

    #198434

    Purpose

    Activity/inhibition assays, 19F-NMR

    Depositor

    Ute Hellmich, Tanja Schirmeister

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    enzymatically cleavable linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eZiPro W61*F

    Plasmid

    #198435

    Purpose

    Activity/inhibition assays, 19F-NMR assignment

    Depositor

    Ute Hellmich, Tanja Schirmeister

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    enzymatically cleavable linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    W61*F

    Promoter

    T7 promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eZiPro W50F

    Plasmid

    #198437

    Purpose

    Activity/inhibition assays, 19F-NMR assignment

    Depositor

    Ute Hellmich, Tanja Schirmeister

    Article

    Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

    Insert

    enzymatically cleavable linked Zika virus NS2B-NS3
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    W50F

    Promoter

    T7 promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-DmPAN2-F1103A-dsRNAres_Y

    Plasmid

    #148044

    Purpose

    Insect Expression of DmPAN2-F1103A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-F1103A-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y

    Plasmid

    #148064

    Purpose

    Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y

    Plasmid

    #148065

    Purpose

    Insect Expression of DmPAN2-del246-249-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del246-249-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmPAN2-del130-133G-T97RH102G-dsRNAres_Y

    Plasmid

    #148033

    Purpose

    Insect Expression of DmPAN2-del130-133G-T97RH102G-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del130-133G-T97RH102G-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y

    Plasmid

    #148034

    Purpose

    Insect Expression of DmPAN2-del246-249-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del246-249-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNAres_Y

    Plasmid

    #148036

    Purpose

    Insect Expression of DmPAN2-del358-467-T97RH102G-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-del358-467-T97RH102G-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pnEA-NpH-HsNot7_9-260_R

    Plasmid

    #147367

    Purpose

    Bacterial Expression of HsNot7_9-260

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot7_9-260 (CNOT7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge