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Str-KDEL_ST-SBP-EGFP

Plasmid

#65264

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Roger Tsien, Erik Rodriguez

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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mCardinal-pBAD

Plasmid

#54800

Purpose

Localization: Protein Expression Vector , Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Use

Tags

6xHis and mCardinal

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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p15_attB(bxb)_lox

Plasmid

#183764

Purpose

Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-vioABECD

Plasmid

#66535

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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