We narrowed to 29,857 results for: REP
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Plasmid#103452PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-339-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-339-3p target (MIR338 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-376c-5p
Plasmid#103500PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-376c-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-376c-5p target (MIR376C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-340-5p
Plasmid#103459PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-340-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-340-5p target (MIR340 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30e-5p
Plasmid#103419PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30e-5p target (MIR30E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-615-5p
Plasmid#103675PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-615-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-615-5p target (MIR615 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMiR-CIP2A(partial)
Plasmid#53705PurposeLuciferase reporter assay for microRNA binding on CIP2A coding sequenceDepositorAvailable SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-377-3p
Plasmid#103501PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-377-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-377-3p target (MIR376C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-34a-5p
Plasmid#103465PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-34a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-34a-5p target (MIR34A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-377-5p
Plasmid#103502PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-377-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-377-5p target (MIR377 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-203a-3p
Plasmid#103336PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-203a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-203a-3p target (MIR203A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-505-3p
Plasmid#103583PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-505-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-505-3p target (MIR505 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1307-3p
Plasmid#103214PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1307-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1307-3p target (MIR1307 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-23a-5p
Plasmid#103372PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-23a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-23a-5p target (MIR23A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-200b-3p
Plasmid#103330PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-200b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-200b-3p target (MIR200B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-5F5-mCherry
Plasmid#73422PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.DepositorInsertPromoter 5F5 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP5F5 (orthogonal T7-lac variant)Available SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
Plasmid#101229PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C, N692A, M694A, Q695A an…PromoterT7Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAPLe4 PCMTD1 PCMTD1(∆ESB-ASB9)
Plasmid#246507PurposeRecombinant protein expression of PCMTD1(∆ESB-ASB9)DepositorInsertProtein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
UseTagsHis-tagExpressionBacterialMutationDeleted amino acids 266-340 in PCMTD1 and replace…PromoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)
Plasmid#242587PurposeA CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).DepositorInsertnlsKateV5, MbBPF-FLAG, FRTNeoFRT
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-DDHD2-30-202-Hygro
Plasmid#238101PurposeExpression of structurally predicted WWE domain of DDHD2 with EGFP on C terminalDepositorInsertDDHD2 (DDHD2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 30-202 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-TRIP12-391-938-EGFP-Hygro
Plasmid#238092PurposeExpression of structurally predicted WWE domain of TRIP12 with EGFP on C terminalDepositorInsertTRIP12 (TRIP12 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 319-938 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro
Plasmid#238096PurposeExpression of structurally predicted WWE domain of HUWE1 with EGFP on C terminalDepositorInsertHUWE1 (HUWE1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-166 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-RNF146(100-182)-myc
Plasmid#196240PurposeN-terminal GST fusion of RNF146(100-182) WWE domain with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of RNF146(100-182) with a TEV protease site between GST and RNF146(100-182) encoding a C-terminal myc tag
UseTagsGST tag and myc tagExpressionBacterialMutationPromoterAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)
Plasmid#229824PurposeA PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tetDepositorInsertLuciferase-P2A-H2A-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-R204A
Plasmid#222196PurposeBacterial expression of mouse PTER-R204A proteinDepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H230F
Plasmid#222195PurposeBacterial expression of mouse PTER-H230F proteinDepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H26F
Plasmid#222192PurposeBacterial expression of mouse PTER-H26F proteinDepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-E169A
Plasmid#222190PurposeBacterial expression of mouse PTER-E169A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-D298A
Plasmid#222191PurposeBacterial expression of mouse PTER-D298A proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H28F
Plasmid#222193PurposeBacterial expression of mouse PTER-H28F proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-mPTER-H201F
Plasmid#222194PurposeBacterial expression of mouse PTER-H201F proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only