We narrowed to 9,520 results for: try

1. pDONR P4-P1R-EYFP

   Plasmid

   #48350

   Purpose

   Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EYFP
   

   Use

   Gateway entry vector

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-V5-mScarlet-I_SV40pA (JDW 968)

   Plasmid

   #224529

   Purpose

   A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

   Plasmid

   #192273

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-CreLite

   Plasmid

   #131782

   Purpose

   Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

   Depositor

   George Eisenhoffer

   Article

   Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

   Insert

   PhyBCreC-P2A-PIF6CreN
   

   Use

   Cre/Lox; Gateway cloning

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUT1159-HQ858703

   Plasmid

   #27749

   Depositor

   Erich Grotewold, John Gray

   Article

   Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

   Insert

   AUX/IAA transcription factor from DV030103
   

   Use

   Entry vector

   Available Since

   March 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUT1455-HQ858749

   Plasmid

   #27831

   Depositor

   Erich Grotewold, John Gray

   Article

   Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

   Insert

   ORPHAN transcription factor from DR801947
   

   Use

   Entry vector

   Available Since

   March 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUT1682-HQ858766

   Plasmid

   #27848

   Depositor

   Erich Grotewold, John Gray

   Article

   Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

   Insert

   GRAS transcription factor from DV163953
   

   Use

   Entry vector

   Available Since

   March 25, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. promoter J23110 (upstream of RBS, mRFP1)

   Plasmid

   #117852

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   medium-high promoter, RBS, mRFP1
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR P4-P1R-mKate2

    Plasmid

    #48344

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Mutation

    It contains a Kozak sequence but no stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid

    #165907

    Purpose

    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8/GW/TOPO-SRF

    Plasmid

    #98618

    Purpose

    Gateway entry TOPO TA cloning vector for SRF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    SRF (Srf Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONRG_P4-P1R:MUM4_0.3Pro_35S

    Plasmid

    #128558

    Purpose

    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning

    Depositor

    George Haughn

    Article

    Dean et al Plant Mol Biol. 2017 Sep;95(1-2):33-50.

    Type

    Empty backbone

    Use

    Gateway promoter entry clone

    Promoter

    MUM4 core promoter with 35S enhancer

    Available Since

    Sept. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pENTR/U6 HDAC4 shRNA

    Plasmid

    #32220

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    shRNA against murine HDAC4 (Hdac4 Mouse)
    

    Use

    RNAi; Gateway u6 entry vector

    Promoter

    human U6

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

16. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75177

    Purpose

    Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Three prime entry vector for multisite gateway th…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

    Plasmid

    #114378

    Purpose

    expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-KA2N-EGFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EGFP and KA2N

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR/U6 HDAC5 shRNA

    Plasmid

    #32222

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    shRNA against mouse HDAC5 (Hdac5 Mouse)
    

    Use

    RNAi; Gateway u6 entry vector

    Promoter

    human U6

    Available Since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

    Plasmid

    #114376

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP, Kv2.1C, and TlcnC

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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