12,468 results

1. pETDUET-GST-AUP1 379-410:Ube2G2

   Plasmid

   #185335

   Purpose

   Used for co-expression of the AUP1 G2BR with UBE2G2 for crystallography

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 aa 379-410, Ube2G2 (AUP1 Mouse, Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EGFP-ShadowY-CLC

   Plasmid

   #193552

   Purpose

   Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   Clathrin light chain A (Clta Mouse)
   

   Use

   Tags

   ShadowY and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

   Plasmid

   #184774

   Purpose

   Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-sgNkx2-1#1/Cre

   Plasmid

   #193221

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Nkx2-1 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgNkx2-1#1 (Nkx2-1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. etnppl-pGEMtEasy

   Plasmid

   #192263

   Purpose

   Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

   Depositor

   Toshihide Yamashita

   Article

   Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

   Insert

   ethanolamine phosphate phospholyase (Etnppl Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-15b(ETNPPL)

   Plasmid

   #192264

   Purpose

   Coding sequence of mouse ETNPPL is inserted in pET-15b vector

   Depositor

   Toshihide Yamashita

   Article

   Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

   Insert

   ethanolamine phosphate phospholyase (Etnppl Mouse)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIS003

   Plasmid

   #194014

   Purpose

   This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

   Depositor

   Timothy Whitehead

   Article

   Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

   Insert

   C16 Ex4 XTR f1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD649-HAsp-mIslr2-COMP5AP-AviTag-9xHis

   Plasmid

   #157627

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   mIslr2 (Islr2 Mouse)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBetaActin-TUBB2a-mScarlet

   Plasmid

   #191324

   Purpose

   Expresses fluorescent labeled beta Tubulin to measure microtubule density

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-mScarlet (Tubb2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid

    #191331

    Purpose

    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBetaActin-IC2N-dCer3

    Plasmid

    #191325

    Purpose

    Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    IC2N-dCer-FRB (Dync1i2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-237

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBetaActin-TUBB2a-Dronpa

    Plasmid

    #191321

    Purpose

    Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-Dronpa (Tubb2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

    Plasmid

    #182888

    Purpose

    Mammalian expression of PVALB mouse mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PVALB mA_BCN14.1.1C9_LC_Mouse Ig Kappa

    Plasmid

    #182889

    Purpose

    Mammalian expression of PVALB mouse mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a

    Plasmid

    #182892

    Purpose

    Mammalian expression of PVALB mouse mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PVALB mAb_BCN14.1.1A2_LC_Mouse Ig Kappa

    Plasmid

    #182893

    Purpose

    Mammalian expression of PVALB mouse mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-Tesmin-BioID2-3xFLAG

    Plasmid

    #186815

    Purpose

    CAG promoter-driven expression of TESMIN-BioID2-3xFLAG

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Insert

    Tesmin (Tesmin Mouse)
    

    Use

    Tags

    BioID2-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pClgn-Tesmin-BioID2-3xFLAG

    Plasmid

    #186816

    Purpose

    Mouse Clgn promoter-driven expression of TESMIN-BioID2-3xFLAG

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Insert

    Tesmin (Tesmin Mouse)
    

    Use

    Tags

    BioID2-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Clgn promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pClgn-Tesmin-TurboID-3xFLAG

    Plasmid

    #186817

    Purpose

    Mouse Clgn promoter-driven expression of TESMIN-TurboID-3xFLAG

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Insert

    Tesmin (Tesmin Mouse)
    

    Use

    Tags

    TurboID-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Clgn promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBa.Halo-KIF3C tail 411-796

    Plasmid

    #193934

    Purpose

    Mammalian expression of Halo-tagged Kif 3C tail 411-796

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3C (Kif3c Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBa.Halo-KIF3C DD 616-796

    Plasmid

    #193938

    Purpose

    Mammalian expression of Halo-tagged Kif 3C DD 616-796

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3C (Kif3c Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBa.GFP-KIF3C DD 616-796

    Plasmid

    #193937

    Purpose

    Mammalian expression of GFP-tagged Kif 3C DD 616-796

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3C (Kif3c Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBa.GFP-KIF3B CC 391-592

    Plasmid

    #193930

    Purpose

    Mammalian expression of GFP-tagged Kif 3B CC 391-592

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3B (Kif3b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-Bid-GFP

    Plasmid

    #191938

    Purpose

    Expression of BID-GFP chimera

    Depositor

    Luca Scorrano

    Insert

    Bid-GFP (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBa.GFP-KIF3C CC 411-615

    Plasmid

    #193935

    Purpose

    Mammalian expression of GFP-tagged Kif 3C CC 411-615

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3C (Kif3c Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBa.Halo-KIF3B CC 391-592

    Plasmid

    #193931

    Purpose

    Mammalian expression of Halo-tagged Kif 3B CC 391-592

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3B (Kif3b Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBa.Halo-KIF3B DD 593-747

    Plasmid

    #193933

    Purpose

    Mammalian expression of Halo-tagged Kif 3B DD 593-747

    Depositor

    Marvin Bentley

    Article

    Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

    Insert

    Kif 3B (Kif3b Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-Tet-On shYap1-4

    Plasmid

    #193670

    Purpose

    Tet inducible knockdown of Yap1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FLAG-mVenusC-14-HP1b Chromo W42A

    Plasmid

    #191399

    Purpose

    W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo W42A
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    W42A

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FLAG-mVenusC-14-HP1b Chromo

    Plasmid

    #191398

    Purpose

    HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge