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pDONR223_EGFR_p.773_774insH

Plasmid

#82855

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Additional H residue inserted between AA 773 and …

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF2_p.T339A

Plasmid

#82770

Purpose

Gateway Donor vector containing ATF2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ATF2 (ATF2 Human)

Use

Gateway entry vector

Mutation

T339A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT3_p.E17K

Plasmid

#82172

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Mutation

E17K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FTH1_p.A161T

Plasmid

#81278

Purpose

Gateway Donor vector containing FTH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FTH1 (FTH1 Human)

Use

Gateway entry vector

Mutation

K88E, L98Q, A161T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR (mut - miR-206 site)

Plasmid

#62576

Purpose

Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Mutation

miR-206 binding site mutated (ACATTCCA --> AAC…

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_39

Plasmid

#60317

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PCSK1 enhancer (PCSK1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_39

Plasmid

#60280

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PCSK1 enhancer (PCSK1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 C299S

Plasmid

#40988

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C299S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2_p53(R280K)_V5

Plasmid

#136525

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53(R280K) (TP53 Human)

Use

Entry vector for gateway cloning

Tags

V5

Mutation

p53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…

Availability

Academic Institutions and Nonprofits only

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pAdeasy-CMV-AE-EF1α-R2

Plasmid

#194928

Purpose

expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis

Depositor

Limei Wang

Article

Wang et al Vaccines (Basel). 2024 Sep 6;12(9):1022.

Inserts

AE
EEF1A1 (EEF1A1 Human)
R2

Use

Adenoviral

Tags

Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c

Mutation

a 48 bp linker (Gly3ser)4 was added between the t…

Promoter

CMV and EF1a

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB2

Plasmid

#23888

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Gs(short)-CASE

Plasmid

#168124

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)

Use

Luciferase

Tags

cpVenus on GNG1

Expression

Mammalian

Mutation

NLuc is inserted at N136/V137 within GNAS

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCX-TRI-2A

Plasmid

#74674

Purpose

mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Inserts

HO1 (HMOX1 Human)
CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R]

Plasmid

#177539

Purpose

Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human). Derived from hybridoma N37A/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ABL1

Plasmid

#23939

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ABL1 (ABL1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-FGFR2

Plasmid

#23904

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR2 (FGFR2 Human)

Use

Gateway donor vector

Mutation

A52D, T525A (compared to GenBank reference sequen…

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-KDR

Plasmid

#23925

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KDR (KDR Human)

Use

Gateway donor vector

Mutation

V297I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-DDR1

Plasmid

#23910

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR1 (DDR1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-WT

Plasmid

#194578

Purpose

Mammalian Expression of mEGFP-CALR WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

Plasmid

#215759

Purpose

HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

Depositor

Dimitrios Wagner

Article

Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

Insert

CD247-truncCD19CAR-GSG (CD19, CD247 Human)

Tags

IgG1 CH3

Expression

Bacterial

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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