We narrowed to 18,870 results for: IRE

1. bbCas9pluspAAA

   Plasmid

   #82581

   Purpose

   The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

   Depositor

   Ivo Huijbers

   Article

   Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. WT Nedd4L

   Plasmid

   #200086

   Purpose

   Nedd4L in pQCXIN vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   Nedd4L (NEDD4L Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQP-CiRISB

   Plasmid

   #138978

   Purpose

   Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR1003

   Plasmid

   #71821

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. NbALFA-TEV219

   Plasmid

   #201487

   Purpose

   TANGO detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbALFA-TEV219
   

   Use

   Tags

   TEV219

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB783

   Plasmid

   #60162

   Purpose

   Firefly luciferase (Promega) driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGPΩTp

   Plasmid

   #130660

   Purpose

   Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep

   Insert

   Ω trimethoprim cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Addition of Ω sites next to trimethoprim cassette…

   Promoter

   dhfr promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-mRor2delta745

   Plasmid

   #22640

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

   Insert

   Ror2 delta 745 (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion C terminal domain

   Promoter

   Available since

   Dec. 18, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pEF1a-mRor2deltaKringle

   Plasmid

   #22630

   Depositor

   Roel Nusse

   Article

   Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

   Insert

   Ror2 delta Kringle (Ror2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of Kringle domain

   Promoter

   Available since

   Nov. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pJH291

    Plasmid

    #172153

    Purpose

    Reporter plasmid for pylBCD variant in pCDF backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para sfGFP.2TAG; Plpp pylS; PproK pylT; AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sfGFP(N39*, Y151*)

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. NbV5-TEV219

    Plasmid

    #201481

    Purpose

    TANGO detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-TEV219
    

    Use

    Tags

    TEV219

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB023k

    Plasmid

    #99219

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    

    Use

    Tags

    Expression

    Mutation

    amber codons at positions 12, 203, and 527 of T7 …

    Promoter

    Ppsp

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC-EsaR-V220A

    Plasmid

    #47645

    Purpose

    pAC-EsaR with a c659t mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-V220A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Valine 220 to Alanine

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. hygII-UT-ABAleon2.15

    Plasmid

    #106983

    Purpose

    ABAleon2.15 in plant expression vector (Hygromycin resistance)

    Depositor

    Julian Schroeder

    Article

    Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

    Insert

    ABAleon2.1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

    Plasmid

    #70211

    Purpose

    1 Part of 4-part switch

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deCFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL0M-S-mClover3-EC18157

    Plasmid

    #137076

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MT21-Ubc9

    Plasmid

    #17273

    Depositor

    Stephen Goff

    Article

    Yueh et al J Virol. 2006 Jan . 80(1):342-52.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pL-rpb1-N488D

    Plasmid

    #10988

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-N488D (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 1462 A to G in the RPB1 ORF resulting in…

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pAT206/Sept7shRNA

    Plasmid

    #38299

    Depositor

    Matthew Krummel

    Article

    Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epu

    Insert

    Septin 7 (Septin7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Pr-deGFP

    Plasmid

    #67743

    Purpose

    Pr promoter expressing deGFP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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