We narrowed to 9,878 results for: EPO

1. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

   Plasmid

   #200123

   Purpose

   Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-Sh-FUCCI-Luc

   Plasmid

   #133364

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Use

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-mScarlet-Control-5'UTR

   Plasmid

   #184637

   Purpose

   Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   mScarlet
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 2, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

   Plasmid

   #170578

   Purpose

   Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDKL5 (CDKL5 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   E854G- please see depositor comments

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-AF8

   Plasmid

   #117803

   Purpose

   mammalian expression of GFP-tagged human centromere protein A

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2000 Aug;25(4):253-61

   Insert

   CENP-A (CENPA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Use

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lyn11-CIBN-GFP

   Plasmid

   #79572

   Purpose

   expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

   Depositor

   Olof Idevall-Hagren, Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   Lyn11
   

   Use

   Tags

   CIBN (CRY2-binding domain) and EGFP

   Expression

   Mammalian

   Mutation

   G8R (please see depositor comments below)

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Use

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIN40C.SFFV.Luciferase.IRES.dTomato

   Plasmid

   #169307

   Purpose

   Constitutive overexpression of Luciferase cDNA with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   IRES-dTomato

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-PNGT3-d2EGFP_CMV-mCherry

    Plasmid

    #213765

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

    Insert

    PNGT#3-d2EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #133786

    Purpose

    Cre-dependent tdTomato fluorescent reporter for cell labeling

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    pAAV-EF1a-DIO-tdTomato-WPRE
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid

    #169552

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-NanoLuc Luciferase - mTagBFP fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTLR repair vector

    Plasmid

    #64322

    Purpose

    Vector for repair of the traffic light reporter (TLR), providing the venus coding region

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    Venus
    

    Use

    Artificial targeting vector

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    none

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHsp83-NLS-HA-2xPCP-2xGFP

    Plasmid

    #71243

    Purpose

    Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    2xPCP-2xGFP
    

    Use

    P element-mediated germline transformation

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Hsp83

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEK19

    Plasmid

    #210578

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    BdBIP1pro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid

    #68426

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three MS2 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid

    #115775

    Purpose

    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    

    Use

    Tags

    EGFP; dsReD

    Expression

    Mammalian

    Mutation

    gp140 uncleaved (1190-91aa, KR > TG; truncated…

    Promoter

    HIV-1 (LTR)

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEK39

    Plasmid

    #210599

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
    

    Use

    Tags

    mRuby3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRZ-mOct4-CNL

    Plasmid

    #65718

    Purpose

    mOct4 reporter plasmid (expresses CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Oct4 promoter and CNL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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