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We narrowed to 16,181 results for: nes

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Showing: 6661 - 6680 of 16181 results

  2. mCherry-7aa-ActA-pEGFP-C1

    Plasmid
    #177406
    Purpose
    To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.
    Depositor
    David Andrews
    Publication
    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    177406-plasmid-map-sequence-id-347979

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  3. pBa.GFP-KIF17 tail 400-1038

    Plasmid
    #134617
    Purpose
    Mammalian expression of mouse KIF1A 400-1038
    Depositor
    Marvin Bentley
    Publication
    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF17 (Kif17 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    WT with deletion of the 399 N-terminal residues.
    Promoter
    Chicken Beta actin
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    134617-plasmid-map-sequence-id-274082

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  4. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126778
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R691A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126778-plasmid-map-sequence-id-259276

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  5. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126762-plasmid-map-sequence-id-410965

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  6. BII-gR-BtW-eSpCas9

    Plasmid
    #133358
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Publication
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9
    Tags
    Flag-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133358-plasmid-map-sequence-id-269429

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  7. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

    Plasmid
    #191500
    Purpose
    GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye
    Depositor
    Michael Granato
    Publication
    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaSR-EGFP-SV40
    Use
    Creation of transgenic fish using tol2 transgenes…
    Promoter
    myo6b
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    191500-plasmid-map-sequence-id-379300

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  10. pDEST-ERBB2-v2

    Plasmid
    #154895
    Purpose
    Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays
    Depositor
    Johanna Ivaska
    Publication
    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERBB2 (ERBB2 Human)
    Tags
    Venus fragment 2 (v2)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154895-plasmid-map-sequence-id-306905

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  12. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Publication
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

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  14. B52 + SMARCAL1 sgSTOP

    Plasmid
    #100715
    Purpose
    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100715-plasmid-map-sequence-id-193651

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  20. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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Showing: 6661 - 6680 of 16181 results