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pDONR223-TRIB2

Plasmid

#23589

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIB2 (TRIB2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-MFSD5_STOP

Plasmid

#161107

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD5 (MFSD5 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A22_STOP

Plasmid

#161217

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A22 (SLC25A22 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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8027_GFP-KrasG12C_2B_puro

Plasmid

#64372

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2B (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-PI4KII

Plasmid

#23503

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4KII (PI4K2A Human)

Use

Gateway donor vector

Mutation

VGSFQLF=>GWFIPAL

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-UCK2

Plasmid

#23863

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

UCK2 (UCK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NME1-NME2

Plasmid

#23353

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME1-NME2 (NME1-NME2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-FLVCR2_STOP

Plasmid

#161070

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

FLVCR2 (FLVCR2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC9C1

Plasmid

#132247

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9C1 (SLC9C1 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A19_STOP

Plasmid

#161429

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A19 (SLC25A19 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-HRAS(G12V)-IRES-p53DD

Plasmid

#233185

Purpose

Retroviral expression of HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-RP6-213H19.1

Plasmid

#23561

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RP6-213H19.1 (STK26 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PAK6

Plasmid

#23833

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK6 (PAK6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-OXSR1

Plasmid

#23847

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

OXSR1 (OXSR1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBMN DHFR-HiBiT / Lyt2

Plasmid

#179731

Purpose

Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

Depositor

James Inglese

Article

Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

Insert

human DHFR-HiBiT and IRES-Lyt-2 (DHFR Human, Mouse)

Use

Retroviral

Tags

HiBiT

Promoter

LTR/MMLV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-NUP62

Plasmid

#23559

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NUP62 (NUP62 Human)

Use

Gateway donor vector

Mutation

S155P

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV-2 NSP5_nostop

Plasmid

#149308

Purpose

Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMX-HA-UbvG08-IRES-GFP

Plasmid

#75030

Purpose

Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Retroviral

Tags

HA and IRES-eGFP

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Available Since

June 30, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-NEK6

Plasmid

#23781

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK6 (NEK6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC35A2_STOP

Plasmid

#161313

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A2 (SLC35A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PBK

Plasmid

#23782

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PBK (PBK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-EPHB6

Plasmid

#23931

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHB6 (EPHB6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC25A46

Plasmid

#131971

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A46 (SLC25A46 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC52A2

Plasmid

#131902

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A2 (GPR172A Human)

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PRKAR2B

Plasmid

#23667

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR2B (PRKAR2B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-SLC27A2_STOP

Plasmid

#161033

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A2 (SLC27A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC29A1

Plasmid

#131927

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC29A1 (SLC29A1 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PRKD3

Plasmid

#23580

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKD3 (PRKD3 Human)

Use

Gateway donor vector

Mutation

Q546R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIP5K2A

Plasmid

#23744

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIP5K2A (PIP4K2A Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-STK16

Plasmid

#23565

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK16 (STK16 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only