We narrowed to 6,838 results for: Proc;
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Plasmid#221536PurposeEGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRsDepositorInserteGFP
ExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOpen-MMLV_RT (mut H)
Plasmid#165546PurposeSingle 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.DepositorInsertMoloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
UseSynthetic BiologyAvailable SinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
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psubCMV-mVEGF-D-WPRE
Plasmid#119226PurposePlasmid encodes full-length (unprocessed) murine VEGF-D to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOpen-MMLV_RT (lack H)
Plasmid#165556PurposeSingle 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.DepositorInsertMoloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
UseSynthetic BiologyAvailable SinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXR004_puroR
Plasmid#219820PurposehU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-phi29pol
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyAvailable SinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psubCAG-WPRE-hVEGF-D-Strept
Plasmid#119227PurposePlasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pL18-ccdB
Plasmid#164898PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.DepositorInsertccdB cassette
TagsHis-tagExpressionBacterialAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HYPC WW domain #2
Plasmid#104232PurposeBacterial expression of WW domain #2 from HYPCDepositorInsertHYPC WW domain #2 (PRPF40B Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
3'dmDR-CMV-CasRx-bGHpolyA
Plasmid#229556PurposeExpresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' endDepositorInsertCasRx
UseCRISPRExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR
Plasmid#221448PurposeExpresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3DepositorInsertswtCas9-NG
mCherry
UseCRISPRExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pR18/pL18-ccdB
Plasmid#164899PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.DepositorInsertccdB cassette
TagsHis-tagExpressionBacterialAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T4 g32
Plasmid#165560PurposeA single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.DepositorInsertT4 gene 32
UseSynthetic BiologyAvailable SinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b-mISG15(GG)
Plasmid#12448DepositorInsertC-terminal processed form of ISG15
TagsHisExpressionBacterialAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAC1819_pCR8-DR-SMN2-2-DR
Plasmid#118653PurposeTransient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway DonorDepositorInsertDR-SMN2-2-DR
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1820_pCR8-DR-RG6-SA-DR
Plasmid#118654PurposeTransient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway DonorDepositorInsertDR-RG6-SA-DR
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
myc-tagged NRP1 (human)
Plasmid#213068PurposeExpress myc-NRP1 in mammalian cellsDepositorInsertneuropilin 1 (NRP1 Human)
TagsMycExpressionMammalianMutationN-terminal myc tag was introduced by a three-step…PromoterT7Available SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNJB80
Plasmid#51518PurposeGateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.DepositorTypeEmpty backboneUseGateway entry vectorAvailable SinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only