We narrowed to 13,293 results for: cDNA

1. pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

   Plasmid

   #190086

   Purpose

   express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

   Insert

   SARS-CoV S (S Synthetic)
   

   Use

   Tags

   Rhodopsin C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

   Plasmid

   #175421

   Purpose

   Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123338

   Purpose

   Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   DAKAP-Venus-cpVenus-FLARE-AKAR
   

   Use

   Tags

   N-terminal 30 amino acids from DAKAP1, Venus, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1129 pcDNA3 GFP FKHR H215R AAA

   Plasmid

   #9034

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR H215R AAA (FOXO1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H215R T24A S256A S319A

   Promoter

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

5. 9E10.mCHOP(S78&81A).pCDNA1/Amp

   Plasmid

   #21902

   Depositor

   David Ron

   Insert

   CHOP (Ddit3 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S78A S81A

   Promoter

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

   Plasmid

   #83453

   Purpose

   Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   polycystin-1 (Pkd1 Mouse)
   

   Use

   Tags

   2xMyc, 3xFlag, and 6xHis

   Expression

   Mammalian

   Mutation

   S4144D

   Promoter

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

   Plasmid

   #70668

   Purpose

   Human mutant of E2F1 lacking amino acids 2 to 126

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 lacking residues 2 to 126 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   lacks amino acids 2-126

   Promoter

   cmv

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

   Plasmid

   #160742

   Purpose

   Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4,K852A)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

   Plasmid

   #79619

   Purpose

   Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   3xFlag-PmCDA1

   Expression

   Mammalian

   Mutation

   D10A for nickase Cas9

   Promoter

   pCMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. (282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160741

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

    Plasmid

    #181814

    Purpose

    Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Muc5b D1 (Muc5b Mouse)
    

    Use

    Tags

    TEV cleavage site followed by 6xHistidine tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

    Plasmid

    #115843

    Purpose

    Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251-Vpx-Q76A
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Q76A

    Promoter

    CMV

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT TO myc Separase Non-Cleavable

    Plasmid

    #59826

    Purpose

    Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

    Plasmid

    #166798

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    LL100EE

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc-ΔD-box-CycA-GFP (525)

    Plasmid

    #39856

    Depositor

    Jonathon Pines

    Article

    den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

    Insert

    Cyclin A2 (CCNA2 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    L2V; Δ destruction box = deletion of aa47-55

    Promoter

    Available Since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Flag-RNF168 delta MIU1/MIU2

    Plasmid

    #133981

    Purpose

    mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU1 and MIU2

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

    Plasmid

    #158604

    Purpose

    Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

    Plasmid

    #187994

    Purpose

    Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    mucin2 D1D2D'D3 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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