We narrowed to 20,307 results for: ACE;
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Plasmid#195960PurposeGateway pME fluorophoreDepositorInsertmApple
UseOtherTagsExpressionMutationPromoterAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCAGAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB24 pDEST crybb1:TagBFP
Plasmid#195987PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2096-GPA-BRET-Biosensor-Retrovirus-TD146
Plasmid#222878PurposeRetroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.DepositorInsertFRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
UseRetroviralTagsMycExpressionMammalianMutationPromoterMSCV LTRAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Mb haloTyrRS C6
Plasmid#160378PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TPromoterAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAHS2 (pBS0249)
Plasmid#185139PurposeFor the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertCAHS2
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Orange chromoprotein
Plasmid#117850PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Orange
UseSynthetic Biology; Escherichia coliTagsExpressionMutation64V,65G, BioBrick sites removedPromoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SV40.eGFP.SV40(polyA)
Plasmid#195558PurposeExpresses eGFP under control of short GFAP promoterDepositorInserteGFP
UseAAVTagsNo tagsExpressionMammalianMutationPromotershort GFAPAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB59 pDEST crybb1:mKate2
Plasmid#195986PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1.cas9-NT
Plasmid#197871PurposeConstitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)DepositorInsertstrancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
UseTagsExpressionBacterialMutationPromoterAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST PUM2
Plasmid#40292DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
gfasPurple chromoprotein
Plasmid#117849PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, gfasPurple
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v4
Plasmid#158032Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-moxGFP-Blast-H2BC11
Plasmid#207757PurposeDonor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
CRISPR-StAR4 (Retro)
Plasmid#222690PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPGK without BbsI cutsitePromoterAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseTagsExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
spisPink chromoprotein
Plasmid#117839PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coliDepositorInsertpromoter, RBS, spisPink
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
asPink chromoprotein
Plasmid#117838PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coliDepositorInsertpromoter, RBS, asPink
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cre stuffer v4
Plasmid#158033PurposeLentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only