We narrowed to 13,049 results for: Mpl

1. pcDNA5-FRT-TO-Nsp16-HF

   Plasmid

   #157725

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp16-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

   Plasmid

   #90015

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-GFP
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag WDR24 pLJM1

   Plasmid

   #46337

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   WDR24 (WDR24 Human)
   

   Use

   Lentiviral

   Tags

   FLag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5-FRT-TO-FH-Nsp14

   Plasmid

   #157718

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGL3 Zscan4 promoter

   Plasmid

   #69249

   Purpose

   Luciferase reporter for Dux4 activation of Zscan4 transcription

   Depositor

   Stephen Tapscott

   Article

   Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

   Insert

   Zscan4 (ZSCAN4 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   The two single nucleotide mismatches found betwee…

   Promoter

   Zscan4

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-GFP-MCC

   Plasmid

   #133307

   Purpose

   "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   microcin-V bacteriocin cassette (cvaC E. coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   N112D (please see depositors comments)

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.3 HA-hTSPAN12

   Plasmid

   #115784

   Purpose

   Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12 (TSPAN12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   multiple silent mutations introduce restriction s…

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO CBX1-APEX2

   Plasmid

   #129642

   Purpose

   Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   CBX1 (CBX1 Human)
   

   Use

   Tags

   1xFLAG and APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TO-Nsp10-HF

   Plasmid

   #157708

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp10-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCDNA4.TO-ORF57-2xCSTREP

    Plasmid

    #136217

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF57
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAPTrap TALEN 2

    Plasmid

    #83369

    Purpose

    Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

    Plasmid

    #86851

    Purpose

    mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP6 (ATP6 Human)
    

    Use

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon corrected

    Promoter

    CMV

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF149 pSOD1G85RAcGFP1

    Plasmid

    #26410

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G85R

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pGAPTrap-TALEN1

    Plasmid

    #83368

    Purpose

    Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    GAPDH_TALEN1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET28a-PTBP1-RRM34

    Plasmid

    #89156

    Purpose

    Recombinant expression of PTBP1-RRM34 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    contains RNA Recognition Motif 3+4

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTetBl-CLIP-Cep131

    Plasmid

    #136875

    Purpose

    Mammalian expression of the centrosomal protein Cep131 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep131 (CEP131 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-E-cadherin promoter

    Plasmid

    #61798

    Purpose

    Luciferase reporter containing the mouse E-cadherin promoter

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5-FRT-TO-Nsp9

    Plasmid

    #157703

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Use

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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