We narrowed to 13,360 results for: Mpl;

1. pAAV-CKIIa-stGtACR2-FusionRed

   Plasmid

   #105669

   Purpose

   Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV Retrograde and AAV1

   Insert

   stGtACR2
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3 Zscan4 promoter

   Plasmid

   #69249

   Purpose

   Luciferase reporter for Dux4 activation of Zscan4 transcription

   Depositor

   Stephen Tapscott

   Article

   Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

   Insert

   Zscan4 (ZSCAN4 Human)
   

   Use

   Luciferase

   Mutation

   The two single nucleotide mismatches found betwee…

   Promoter

   Zscan4

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSN068

   Plasmid

   #101749

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available Since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-DHFR F[3]-C (LEU2)

   Plasmid

   #177796

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[3]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-mScarlet-Puro-H2BC11

   Plasmid

   #207761

   Purpose

   Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. ALFA-bArr1

   Plasmid

   #201495

   Purpose

   Expression of the beta-arrestin1 containing a N-terminal ALFA tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   ALFA-bArr1 (ARRB1 Human)
   

   Tags

   ALFA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBpaRS3tRNA

   Plasmid

   #197102

   Purpose

   To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

   Insert

   TyrRS variant, amber suppressor tRNA
   

   Expression

   Bacterial

   Mutation

   Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYTRW09K_0T5

   Plasmid

   #177281

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   Ptet-tetA(C)
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Expression

   Bacterial

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. OPEN HLA-A*02:01-BirA

   Plasmid

   #215569

   Purpose

   E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN HLA-A*02:01 (HLA-A Human)
   

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   Glycine 120 to Cysteine

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-6P-1 p37deltaSEP

    Plasmid

    #113501

    Purpose

    Bacterial expression of GST-tagged p37 lacking the SEP domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 140-206

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid

    #127370

    Purpose

    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-U6-UL29-egfp-U3-UL8

    Plasmid

    #166694

    Purpose

    To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

    Depositor

    Yujia Cai

    Article

    Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

    Insert

    UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)
    

    Use

    Lentiviral

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT1-24X-NOG(AtUBI10)

    Plasmid

    #202016

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TK-GFP-Nluc-P2A-neo-TK

    Plasmid

    #134896

    Purpose

    Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Insert

    TK-GFP-Nluc-P2A-Neo-TK
    

    Use

    Cryptosporidium expression

    Promoter

    Cryptosporidium actin and enolase

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lamp1-mCherry-RUSH

    Plasmid

    #202799

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    Lamp1 (Lamp1 Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT-TO-Nsp16-HF

    Plasmid

    #157725

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16-HF (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEGFP Abi1

    Plasmid

    #74905

    Purpose

    Expresses human Abi1 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

    Insert

    Abi1 (ABL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGB_YFP-MiniTurboID

    Plasmid

    #222433

    Purpose

    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

    Depositor

    Dominique Bergmann

    Article

    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

    Insert

    MiniTurboID (BirA mutant)
    

    Use

    Golden gate / green gate compatible cloning vector

    Tags

    Linker and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP10-EGFP

    Plasmid

    #210369

    Purpose

    Expresses FKBP10 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    FKBP10 (FKBP10 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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