We narrowed to 9,184 results for: Pol;

1. pFB1.HMBP.PrS.SUZ12

   Plasmid

   #125162

   Purpose

   Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Polycomb protein SUZ12 (SUZ12 Human)
   

   Use

   Baculovirus expression vector

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-Puro-Flag-IKBKE

   Plasmid

   #15292

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

3. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

   Plasmid

   #208047

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Mutation

   Cys>Ala mutations in conserved RING domain

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-Neo-Flag-IKBKE

   Plasmid

   #15265

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKBKE (IKBKE Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pBabe-Puro-cRAF-W22

   Plasmid

   #15270

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   CRAF (RAF1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   C-terminal deletion

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-N-Myc_DED/AAA USP7

   Plasmid

   #131253

   Purpose

   Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert USP7 aspart…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBactin-Nedd1-mOrange2-hCM1

   Plasmid

   #196863

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

   Plasmid

   #208943

   Purpose

   A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

   Plasmid

   #107576

   Purpose

   B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   GFP+
   

   Use

   Synthetic Biology

   Tags

   B. megaterium DSM319 xylA leader sequence (MTSSKI…

   Expression

   Bacterial

   Mutation

   F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPL3-hRPH3A_c.1853Gmut

    Plasmid

    #190479

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Expression

    Mammalian

    Mutation

    c.1853A>G

    Promoter

    SV40

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

    Plasmid

    #42650

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV/TO

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 ATP5O(5’UTR)-FF

    Plasmid

    #85486

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti.PGK.blast-Flag-MFF-S155,172D

    Plasmid

    #74442

    Purpose

    Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pBabe-Neo-Myr-Flag-IKBKE

    Plasmid

    #15267

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

    Plasmid

    #208949

    Purpose

    A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

    Plasmid

    #208948

    Purpose

    A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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